[Histonet] Help for identifying the blue stained structure

Marvin Hanna mhanna <@t> histosearch.com
Sun May 18 16:47:21 CDT 2014

Hi Rui,

You uploaded a tif image and a number of browsers don't support tif 
images. Jpeg, gif and png images are the best image formats to use 
because they are universally supported. I converted your image to a jpeg 
image and posted it at:


Best Regards,

Marvin Hanna

On 05/17/2014 06:39 PM, Rui TAHARA wrote:
> Hello,
> I have an embryonic sample that
> decalcified, paraffin embedded, and stained with Mallory Trichrome (Aniline
> Blue, Orange G, Acid Fuchsin). I will upload the image in the Histonet Images. This
> is a cranial region where the bone is being resorbed, so I expected to see the
> bone (dark blue in trabecular), and red blood, and adjacent white spaces that
> is being resorbed. Instead, there is a very uniform, granular structure stained
> with blue without any nuclei at the resorbed regions. This structure looks like
> almost crystal or some kind of secretion leakage from the ossifying bone. I
> need a help to identify this blue stained things. I don't think this is osteoid,
> because at later stage embryos, there is no bone at this region.
> If you have some suggestions for other
> stain to identify this blue thing, or help identifying this, I really
> appreciate it.
> Thank you in advance,
> Rui
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