[Histonet] Help for identifying the blue stained structure

[Rui TAHARA]

Hello, 
I have an embryonic sample that
decalcified, paraffin embedded, and stained with Mallory Trichrome (Aniline
Blue, Orange G, Acid Fuchsin). I will upload the image in the Histonet Images. This
is a cranial region where the bone is being resorbed, so I expected to see the
bone (dark blue in trabecular), and red blood, and adjacent white spaces that
is being resorbed. Instead, there is a very uniform, granular structure stained
with blue without any nuclei at the resorbed regions. This structure looks like
almost crystal or some kind of secretion leakage from the ossifying bone. I
need a help to identify this blue stained things. I don’t think this is osteoid,
because at later stage embryos, there is no bone at this region. 

If you have some suggestions for other
stain to identify this blue thing, or help identifying this, I really
appreciate it. 

 

Thank you in advance, 

 

Rui