[Histonet] RE: Histonet Digest, Vol 126, Issue 9
Zimmerman, Billie
BZIMMERM <@t> gru.edu
Thu May 8 12:16:49 CDT 2014
I'm a medical technologist working in anatomic pathology and I take offense to your post, Bernice.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, May 08, 2014 1:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 126, Issue 9
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Today's Topics:
1. (no subject) (cynthia haynes)
2. Award Spotlight - Ann Preece Scholarship (Elizabeth Chlipala)
3. RE: Edit protocol for Shandon Gemini Varistainer
(Stephenson, Sheryl)
4. (no subject) (histogirl4 <@t> gmail.com)
5. Slide Ovens (Amber McKenzie)
6. RE: Edit protocol for Shandon Gemini Varistainer (Laurie Colbert)
7. Dako Hercept Kits (Nutter, James E)
8. Unsubscribe from emails (awill.imdpathlab <@t> yahoo.com)
9. Cryo Macrotome (Cameron Nowell)
10. cryoprotection and anti.freeze (Christina Kreutzer)
11. Histology Service Supervisory Position open (Lesley Bechtold)
12. Fisher Embedding Meda (scott munday)
13. Position open in Boston Suburb in Pharma/Biotech (Jason Blaine)
14. CAP at NU (Bernice Frederick)
----------------------------------------------------------------------
Message: 1
Date: Wed, 7 May 2014 09:52:41 -0700 (PDT)
From: cynthia haynes <naje1972 <@t> yahoo.com>
Subject: [Histonet] (no subject)
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1399481561.31655.YahooMailNeo <@t> web160604.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
My name is Cynthia James; I work in a histopathology lab?in Melrose Park Illinois. We are told to change the solution(2-Methyl Butane) which?we use in the Histobath to freeze tissues for frozen sections to something less flammable. I remember a solution was?discussed that can be used instead of the?which is less flammable than the ?IsoPentane that we are currently using.
Would someone contact at 708-681-3200 ext:2012 or 2036 and tell me more about this solution.I am available from 5:00 am to 1:30 pm monday-friday central time, if you know the company that is selling this solution please let me know.
?
Thank you in advance
Cynthia James
?
P.S I can also be reached at this email address naje1972 <@t> yahoo.com or Cynthia_Haynes-James <@t> luhs.org
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Message: 2
Date: Wed, 7 May 2014 11:34:43 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] Award Spotlight - Ann Preece Scholarship
To: "'histonet <@t> lists.utsouthwestern.edu'
(histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE019C79E05A9E <@t> SBS2K8.premierlab.local>
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Awards Spotlight - Ann Preece Scholarship
PolySciences, Inc. sponsors this $1000 award on a reimbursement basis. Polysciences has been sponsoring this award since 2004, Ann Preece passed in 2003. She was born November 12, 1923 in Boston, Massachusetts. She worked at the pathology lab at Scripps Memorial Hospital and authored A Manual for Histological Technician which was published in three editions by Little, Brown & Co. I'm old and lucky enough to have a copy of her book in my lab and I'm sure many others out there may also have a copy.
Award Criteria:
This award will be given annually to a qualified applicant who has excelled in working with calcified/decalcified bone, other materials such as stents in vascular research or bioengineering development. The specimens can be human or animal in origin. This award will support educational or professional certification programs that are intended to:
1. Enhance techniques and methods presently utilized in the nominee's institution, and/or 2. Support the study of new techniques under consideration for adoption in the nominee's laboratory, and/or 3. Support continuing education or certification.
The past recipients of this award are Sarah Mack, Carol Bain, Damien Laudier, Robert Skinner, Vicki Kalscheur, Linda Jenkins, Jack Radcliff, Helen Wimer, Gayle Callis and Nancy Troiano. I know anyone who reads this list will know that these individuals are recognized as the experts in the field with respects to bioengineered constructs, stents, undecalcifed and decalcified bone. If you know of anyone who excels and is passionate in this specialized area of histology then nominate them for this award. http://nsh.org/scholarships-awards/education/individual%20scholarships
Happy Hump Day!
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com<mailto:liz <@t> premierlab.com>
www.premierlab.com<http://www.premierlab.com/>
March 10, 2014 is Histotechnology Professionals Day
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
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Message: 3
Date: Wed, 7 May 2014 18:14:38 +0000
From: "Stephenson, Sheryl" <sheryl.stephenson <@t> ag.state.nj.us>
Subject: RE: [Histonet] Edit protocol for Shandon Gemini Varistainer
To: Deloris Carter <delsuec <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D31AFEA64FC23047B230ED81C0E4AC017A7BDE <@t> CLOVER.ag.state.nj.us>
Content-Type: text/plain; charset="utf-8"
Lol, I had the same problem. Yes the manual does not thoroughly explain this. U have to delete the protocol from the active stains list, edit the protocol and make sure reagents are linked to the correct buckets, then add the protocol back to the active stain list.
I'm not sitting in front of my stainer right now to walk you through screen by screen but that is the whole idea behind it. Delete it, edit it, then add it back. (When you delete it, it's not deleted from the stainer its only deleted from being an active stain protocol.)
I hope this helps a little. If you need further help, let me know I'll go to my machine and get the step by step info for you. You can also call their customer service # and they should be able to walk u thru it.
Thanks,
Sheryl Stephenson
Histotechnologist
NJ Department of Agriculture
Animal Health Diagnostic Labs
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Deloris Carter
Sent: Wednesday, May 07, 2014 12:48 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Edit protocol for Shandon Gemini Varistainer
Can anyone tell me how to edit a program on the Gemini Varistainer? I need to add heat steps to our deparaffinization protocol, but the manual is not very helpful.
Thanks,
Deloris Carter, HT(ASCP)
Shawnee Mission Medical Center
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------------------------------
Message: 4
Date: Wed, 7 May 2014 18:05:52 +0000
From: <histogirl4 <@t> gmail.com>
Subject: [Histonet] (no subject)
To: " histonet <@t> lists.utsouthwestern.edu "
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <536a78e1.6572320a.47ec.799b <@t> mx.google.com>
Content-Type: text/plain; charset="utf-8"
Job Opportunity: Pathology Associates Of Greenville Texas is seeking a full-time Histotehnologist/Histotechnichian, to join out practice.
We are a free standing anatomic pathology laboratory with hospital affiliations, staffed by five pathologists and two full-time Histotechnologists/Histotechnicians. Our laboratory is fully automated. We preform routine surgical pathology, few non-gyn cytology, special stains (Histochemistry) and Immunohistchemistry (IHC).
Requirements for the successful applicant: Strong work ethics- Excellent Interpersonal Skills and willing to assist in Frozen sections and Maintain Policies and Procedures of The Histology Laboratory. Candidates are preferably ASCP Certified/Eligible.
Interested applicants may fax their resume at 903-454-1716
E-mail address: ebrmo <@t> yahoo.com
Sent from Windows Mail
------------------------------
Message: 5
Date: Wed, 7 May 2014 18:34:01 +0000
From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] Slide Ovens
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5A33C952BB67F4468AF1F36D739212BC011248A270 <@t> JERRY.Gia.com>
Content-Type: text/plain; charset="utf-8"
Does anyone use the Thermo High-Capacity Section Dryer Item number B3120202? I've never seen it in person and I was debating on getting it vs the Cardinal one: M7289-20 SD-II-120 Slide dryer II. Any advice? Thanks!
------------------------------
Message: 6
Date: Wed, 7 May 2014 18:31:54 +0000
From: Laurie Colbert <lcolbert <@t> pathmdlabs.com>
Subject: RE: [Histonet] Edit protocol for Shandon Gemini Varistainer
To: Deloris Carter <delsuec <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<12ECD7346266D74691EC2BFC75285E452F4748CA <@t> BFL323E10.pathmdlabs.local>
Content-Type: text/plain; charset="utf-8"
Call Thermo and talk to Sherry Anderson at x562. She is awesome! She just walked me through some programming issues this morning.
Thermo (800) 522-7270 x562
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Deloris Carter
Sent: Wednesday, May 07, 2014 9:48 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Edit protocol for Shandon Gemini Varistainer
Can anyone tell me how to edit a program on the Gemini Varistainer? I need to add heat steps to our deparaffinization protocol, but the manual is not very helpful.
Thanks,
Deloris Carter, HT(ASCP)
Shawnee Mission Medical Center
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------------------------------
Message: 7
Date: Wed, 7 May 2014 17:29:11 -0400
From: "Nutter, James E" <James.E.Nutter <@t> questdiagnostics.com>
Subject: [Histonet] Dako Hercept Kits
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AE373B3D88B43B438369A0AE2354DD9E0B8B08C5 <@t> qdcws0794.us.qdx.com>
Content-Type: text/plain; charset="us-ascii"
Hello everyone,
Has anyone had a problem with recent lots of Dako Hercept Kits?
Thank you,
James E. Nutter Jr. BS, HT & QIHC(ASCP)
Quest Diagnostics Nichols Institute | Histology| 14225 Newbrook Dr.| Chantilly Va. USA | phone 703.802.6900 x65782| fax 703.802.7191| | James.E.Nutter <@t> QuestDiagnostics.com
<mailto:James.E.Nutter <@t> QuestDiagnostics.com> | www.NicholsInstitute.com <http://www.nicholsinstitute.com/>
______________________________________________________________________
The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited.
------------------------------
Message: 8
Date: Wed, 7 May 2014 17:10:36 -0700 (PDT)
From: "awill.imdpathlab <@t> yahoo.com" <awill.imdpathlab <@t> yahoo.com>
Subject: [Histonet] Unsubscribe from emails
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1399507836.10559.YahooMailAndroidMobile <@t> web126103.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Can you unsubscribe me from all the emails or tell me where to go to do so, please.?
Sent from Yahoo Mail on Android
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Message: 9
Date: Thu, 08 May 2014 02:49:11 +0000
From: Cameron Nowell <Cameron.Nowell <@t> monash.edu>
Subject: [Histonet] Cryo Macrotome
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<69A36BE875B3674093D86BD7E716D57C0E4C57 <@t> PRK-MBX-04.vcp.local>
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Hi List,
We are looking at doing whole mouse sectioning for imaging on a beta imaging system. Does anyone know of any other cryo macrotome systems other than the Leica CM3600 (http://www.leicabiosystems.com/specimen-preparation/sectioning/cryosectioning/details/product/leica-cm3600-xp/) ?
It is way too large for what we need (only mice/rats) and way too expensive.
Alternatively is there a standard macrotome out there for doing this sort of sectioning? What do people use for cutting full slices of human brain?
Cheers
Cam
Cameron J. Nowell
Research Facilities Manager
Monash Institute of Pharmaceutical Sciences Monash University
399 Royal Parade
(Mail address: 381 Royal Parade)
Parkville, VIC, 3052
Australia
Email: cameron.nowell <@t> monash.edu<mailto:cameron.nowell <@t> monash.edu>
Mobile: +61 422882700
Office: +61 9903 9587
LinkedIn: Profile<http://au.linkedin.com/pub/cameron-nowell/23/57/884/>
Research Gate: Profile<http://www.researchgate.net/profile/Cameron_Nowell>
------------------------------
Message: 10
Date: Thu, 8 May 2014 11:27:32 +0200
From: Christina Kreutzer <christina.kreutzer01 <@t> gmail.com>
Subject: [Histonet] cryoprotection and anti.freeze
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CANS3E9sTpvycu4hdr3d-GzpxhUUzp9-dgav2oWdiQOJybxd7ag <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Dear Members,
I was wondering what cryoprotection protocols you are using for perfusion fixed tissues (30% succrose, 1:1 Tissue Tek/PBS etc). Why are you using what you are using and did you ever have any problems. Second, I wanted your opinion concerning leaving tisse in cryoprotection solution for several months and antibody reactivity and/or if somebody ever had problems with antibody reactivity on CPS (Glycerin, Ethylene Glycol, PO4 buffer and water).
Thanks in advance
Christina
------------------------------
Message: 11
Date: Thu, 8 May 2014 12:45:51 +0000
From: Lesley Bechtold <Lesley.Bechtold <@t> jax.org>
Subject: [Histonet] Histology Service Supervisory Position open
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<08997064E2075247A4DD5A035DB51CDF64F89F77 <@t> jaxbhexms01.jax.org>
Content-Type: text/plain; charset="us-ascii"
We have an opening for a Histology Service Supervisor position. Please see the posting below.
Supervisor, Histology Services
The Histology Service Supervisor is responsible for day-to-day oversight of the Histology Service. This includes supervision of facility staff, work flow oversight and contributing to the completion of the overall workload in the Histology Service. This includes supervision of facility staff, providing training and guidance as needed. This position also includes carrying out routine and advanced histological procedures and contributing to completion of the overall workload in the department.
Required Skills:
* Excellent verbal and written communication skills.
* Must be able to communicate effectively with others verbally and in writing to clearly and effectively exchange information specifically in the development of protocols and interpretation of data.
* Current computer skills including use of spreadsheet, word processing, e-mail and internet tools. Must be able to utilize electronic databases and information management systems to record, monitor and analyze workflow.
* Must be able to function effectively in a team environment and work with frequent changes in schedules and priorities.
Required Experience:
* Bachelor's degree in a biological science with HT (ASCP) or HTL (ASCP) certification and eight to ten (8-10) years of histology experience as a certified Histotechnologist.
* Experience with histological techniques using animal models is desirable.
* Certification in immunohistochemistry (QIHC) is required although equivalent experience (2 or more years) in immunohistochemical techniques may be considered.
* Three to five (3-5) years of supervisory or lead experience.
* Supervisory or lead experience in a clinical or pharmaceutical setting is desirable.
Interested applicants should apply on line at www.jax.org/careers<http://www.jax.org/careers>, referring to job posting #4377 with a cover letter and resume.
Lesley S. Bechtold
Senior Manager, Histopathology Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322 (phone)
207-288-6325 (fax)
The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible.
------------------------------
Message: 12
Date: Thu, 8 May 2014 10:05:26 -0400
From: scott munday <mundayscott <@t> gmavt.net>
Subject: [Histonet] Fisher Embedding Meda
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAP5y=Q0QgUwkd1fee-J03_JqEad6EkOaDs7LjfKcOzoSx3xhRA <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
We have some extra Fisher Scientific Embedding Media for sale. It's new in the box and I have 2 cases. Each case has 4 five pound cartons in it.
If anyone needs it, I have posted a link to the listing on Ebay below.
http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=151297973845
--
Scott Munday
Munday Scientific Instrument Service
90 Misha Lane
Sanford, NC 27330
Phone: 919-775-5596
Fax: 919-776-9566
------------------------------
Message: 13
Date: Thu, 8 May 2014 15:56:32 +0000
From: Jason Blaine <jblaine <@t> astrixinc.com>
Subject: [Histonet] Position open in Boston Suburb in Pharma/Biotech
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A115DD9FF6F4A14D8DCF77948DCE580C1A5C8BBC <@t> MBX021-W3-CA-2.exch021.domain.local>
Content-Type: text/plain; charset="Windows-1252"
Hi. I am a scientific/pharma recruiter. One of my pharmaceutical/biotech customers is seeking to hire a contract IHC specialist. This is great opportunity to move out of a hospital setting or a processing lab (LabCorp, Quest, etc.) and in to a research/industry environment. Your efforts will support research and development of cancer and infectious disease drug candidates.
This position is full-time, M-F, 9 to 5 (approx) and located in Waltham, MA just off of 95.
If you are interested please send me a copy of your resum? (in MS Word format) to jblaine <@t> astrixinc.com. Please also include your desired compensation. If this position is not right for you but you know of colleagues that may be more appropriate please forward them this information. Thanks!
Below is a list of responsibilities:
* Performs microtomy of formalin fixed paraffin embedded (FFPE) tissues.
* Provides tissue processing and embedding support to all projects and programs including Discovery, Biologics, Safety Assessment, Cancer and Infectious Diseases.
* Reviews and interprets Immunohistochemical and H&E stained tissue sections by light microscopy and fluorescence microscopy for Cancer and Safety Assessment projects.
* Provides biomarker support by performing routine staining of tissue specimens utilizing automated and manual IHC procedures.
* Implement quantitative image analysis for dual stained IHC slides.
* Primary work interactions with the pathologist as well as the in vivo bioscientists
* Prepares data presentations to project meetings
* Work with histopathology management to lead the implementation of the quantitative analysis of image data for IHC including localization and expression patterns in rodent preclnical tissue.
* Document preparation including IHC validation reports and SOPs
Jason Blaine
Managing Director
Email: jblaine <@t> AstrixInc.com
Creating Value and Trust in the Business of Science?
CONFIDENTIALITY NOTICE: The information contained in this message may be CONFIDENTIAL and is for the intended addressee only. Any unauthorized use, dissemination of the information, or copying of this message is prohibited. If you are not the intended addressee, please notify the sender immediately and delete this message.
------------------------------
Message: 14
Date: Thu, 8 May 2014 16:22:13 +0000
From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: [Histonet] CAP at NU
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<62C639732D3F274DACED033EBDF6ADAF2F0A17B4 <@t> evcspmbx2.ads.northwestern.edu>
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Oh yay! CAP is visiting us today. Why do they send an MT to do histo? And a snarky one at that......
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu<mailto:b-frederick <@t> northwestern.edu>
------------------------------
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