[Histonet] cryoprotection and anti.freeze

[Christina Kreutzer]

Dear Members,
I was wondering what cryoprotection protocols you are using for perfusion
fixed tissues (30% succrose, 1:1 Tissue Tek/PBS etc). Why are you using
what you are using and did you ever have any problems. Second, I wanted
your opinion concerning leaving tisse in cryoprotection solution for
several months and antibody reactivity and/or if somebody  ever had
problems with antibody reactivity on CPS (Glycerin, Ethylene Glycol, PO4
buffer and water).

Thanks in advance
Christina