[Histonet] Re: Cornflaking

[joe joe]

Why doesn't someone call Kellogg's ?




> On Mar 13, 2014, at 12:10 PM, "Beth Cox" <bethcoxx <@t> gmail.com> wrote:
> 
> Sharon & other Histonetters,
> 
> Cornflaking is literally microscopic air trapped under the coverslip. It
> doesn't have anything to do with poor dehydration and trapped water. Thus
> it can be caused by partial drying out before the coverslipping.
> 
> The things you need to look at to eradicate the problem is:
> 1.  Keep the slides wet before coverslipping (obviously)
> 2.  Consider increasing the amount of xylene deposited on the slide for
> tape coverslippers.
> 3.  For glass coverslippers, consider increasing the xylene and increasing
> or changing the mounting media.
> 
> Cornflaking tends to happen more on slides/sections with rough "topography"
> on the section (the more rough it is, the more nooks & crannies to trap the
> air).  So anything that would give the section a more rough surface would
> increase the tendency to cornflake; such as: section lifting. section
> thickness, even chatter in the sections.  Think about things that would
> affect the section - for example, are you using a different brand of blade
> on the microtome?
> 
> Hope this helps,
> 
> Beth Cox, HTL/SCT(ASCP)QIHC
> 
> 
> 
> 
> Message: 15
> Date: Thu, 13 Mar 2014 11:59:06 -0400
> From: srishan <@t> mail.holyname.org
> Subject: Re: [Histonet] RE: Cornflaking artifact
> To: "HERRINGTON, SHEILA" <SHEILA.HERRINGTON <@t> interiorhealth.ca>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
>        <histonet <@t> lists.utsouthwestern.edu>,    Sharon Scalise
>        <SSCALISE <@t> beaumont.edu>,
> histonet-bounces <@t> lists.utsouthwestern.edu,
>        'Laurie Colbert' <lcolbert <@t> pathmdlabs.com>
> Message-ID:
>        <
> OF46844F54.2F7FC911-ON85257C9A.00575CB9-85257C9A.0057CEBC <@t> holyname.org>
> Content-Type: text/plain; charset="US-ASCII"
> We had this problem several years ago.  We  were using the sakura tapes
> with the coverslipper.
> We did the following:
> Last three alcohols were changes frequently.
> Slides should be not dry when loading on coverslipper.
> If you could load two racks at a time, only load one.  By this way the
> slides in the second rack will not dry out.
> Finally, change the tapes from sakura to Mercedes Medical tapes.
> Mala
> Nirmala Srishan
> Holy Name Medical Center
> 
> 
> From:   "HERRINGTON, SHEILA" <SHEILA.HERRINGTON <@t> interiorhealth.ca>
> To:     'Laurie Colbert' <lcolbert <@t> pathmdlabs.com>, Sharon Scalise
> <SSCALISE <@t> beaumont.edu>, "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Date:   03/13/2014 11:43 AM
> Subject:        [Histonet] RE: Cornflaking artifact
> Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu
> 
> We also have recently started to see this artifact more than ever before,
> and nothing in our process has changed.  We have tried everything to
> correct to no avail.  Wonder if it is possible to be a change in some type
> of supply, either xylene or coverslipping film.  Something has changed but
> am at a loss as to what.
> 
> Sheila Herrington
> Technical Lead Histopathology and Immunohistochemistry
> Kelowna General Hospital
> 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
> 250-862-4300 ext 7587 or 7510
> Sheila.herrington <@t> interiorhealth.ca
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [
> mailto:histonet-bounces <@t> lists.utsouthwestern.edu<histonet-bounces <@t> lists.utsouthwestern.edu>]
> On Behalf Of Laurie
> Colbert
> Sent: Thursday, March 13, 2014 6:30 AM
> To: Sharon Scalise; histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Cornflaking artifact
> You will also see the cornflaking if your tissue is lifting off of the
> slide at all.  We used to get this more often on hard, decal specimens
> than on other specimens.  We used the film to coverslip.  If you remove
> the film from the problem slides and recoverslip conventionally with extra
> mountant and glass coverslips, I'm sure you will not see the artifact.
> Laurie Colbert
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [
> mailto:histonet-bounces <@t> lists.utsouthwestern.edu<histonet-bounces <@t> lists.utsouthwestern.edu>]
> On Behalf Of Sharon
> Scalise
> Sent: Wednesday, March 12, 2014 8:00 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Cornflaking artifact
> I am looking for help with "cornflaking" (tiny, brown dry spots under
> coverslip)artifact.  We have been using fresh xylene on our stainer and
> coverslipper, cleaned and wiped all containers dry before filling, tried
> different lots of coverslipping film and had service on our coverslipper
> to make sure it was functioning properly, including the xylene drip.  We
> continue to have this artifact and it is driving us crazy.  It is sporadic
> with no pattern of tissue type or placement on the slide.  Sometimes it
> lands on tissue other times not.  Most of the time when we remove the
> coverslip and re-coverslip it goes away (I am assuming because the acetone
> removes any minute amounts of water that may be present).  We just cannot
> figure out where the water is coming from.  Has anyone seen this artifact
> while using the drying step on the prisma stainer?  We just recently
> started using the drying on some slides and I am thinking maybe it is
> causing humidity???  I cannot say for a fact that our "cornflaking"
> started at the same time, but it is suspicious. HELP!!!!!
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