[Histonet] Re: Cornflaking

Thu Mar 13 13:31:37 CDT 2014

We are all putting out what worked for us.  You should  try some of these 
suggestions and see what works for you.  The suggestion for improving poor 
dehydration was suggested by technical support  from Sakura.  Cornflaking 
was irritating so many of our pathologist, we had to get technical support 
from Sakura to see how this could be resolved.  In OUR hospital changing 
the tape worked!!!  Good Luck.!!!

Mala

Nirmala Srishan
Holy Name Medical Center.

From:   joe joe <powderhound34 <@t> hotmail.com>
To:     Beth Cox <bethcoxx <@t> gmail.com>
Cc:     "histonet <@t> lists.utsouthwestern.edu" 
<histonet <@t> lists.utsouthwestern.edu>
Date:   03/13/2014 02:20 PM
Subject:        Re: [Histonet] Re: Cornflaking
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu

Why doesn't someone call Kellogg's ?

> On Mar 13, 2014, at 12:10 PM, "Beth Cox" <bethcoxx <@t> gmail.com> wrote:
> 
> Sharon & other Histonetters,
> 
> Cornflaking is literally microscopic air trapped under the coverslip. It
> doesn't have anything to do with poor dehydration and trapped water. 
Thus
> it can be caused by partial drying out before the coverslipping.
> 
> The things you need to look at to eradicate the problem is:
> 1.  Keep the slides wet before coverslipping (obviously)
> 2.  Consider increasing the amount of xylene deposited on the slide for
> tape coverslippers.
> 3.  For glass coverslippers, consider increasing the xylene and 
increasing
> or changing the mounting media.
> 
> Cornflaking tends to happen more on slides/sections with rough 
"topography"
> on the section (the more rough it is, the more nooks & crannies to trap 
the
> air).  So anything that would give the section a more rough surface 
would
> increase the tendency to cornflake; such as: section lifting. section
> thickness, even chatter in the sections.  Think about things that would
> affect the section - for example, are you using a different brand of 
blade
> on the microtome?
> 
> Hope this helps,
> 
> Beth Cox, HTL/SCT(ASCP)QIHC
> 
> 
> 
> 
> Message: 15
> Date: Thu, 13 Mar 2014 11:59:06 -0400
> From: srishan <@t> mail.holyname.org
> Subject: Re: [Histonet] RE: Cornflaking artifact
> To: "HERRINGTON, SHEILA" <SHEILA.HERRINGTON <@t> interiorhealth.ca>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
>        <histonet <@t> lists.utsouthwestern.edu>,    Sharon Scalise
>        <SSCALISE <@t> beaumont.edu>,
> histonet-bounces <@t> lists.utsouthwestern.edu,
>        'Laurie Colbert' <lcolbert <@t> pathmdlabs.com>
> Message-ID:
>        <
> OF46844F54.2F7FC911-ON85257C9A.00575CB9-85257C9A.0057CEBC <@t> holyname.org>
> Content-Type: text/plain; charset="US-ASCII"
> We had this problem several years ago.  We  were using the sakura tapes
> with the coverslipper.
> We did the following:
> Last three alcohols were changes frequently.
> Slides should be not dry when loading on coverslipper.
> If you could load two racks at a time, only load one.  By this way the
> slides in the second rack will not dry out.
> Finally, change the tapes from sakura to Mercedes Medical tapes.
> Mala
> Nirmala Srishan
> Holy Name Medical Center
> 
> 
> From:   "HERRINGTON, SHEILA" <SHEILA.HERRINGTON <@t> interiorhealth.ca>
> To:     'Laurie Colbert' <lcolbert <@t> pathmdlabs.com>, Sharon Scalise
> <SSCALISE <@t> beaumont.edu>, "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Date:   03/13/2014 11:43 AM
> Subject:        [Histonet] RE: Cornflaking artifact
> Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu
> 
> We also have recently started to see this artifact more than ever 
before,
> and nothing in our process has changed.  We have tried everything to
> correct to no avail.  Wonder if it is possible to be a change in some 
type
> of supply, either xylene or coverslipping film.  Something has changed 
but
> am at a loss as to what.
> 
> Sheila Herrington
> Technical Lead Histopathology and Immunohistochemistry
> Kelowna General Hospital
> 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
> 250-862-4300 ext 7587 or 7510
> Sheila.herrington <@t> interiorhealth.ca
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [
> mailto:histonet-bounces <@t> lists.utsouthwestern.edu
<histonet-bounces <@t> lists.utsouthwestern.edu>]
> On Behalf Of Laurie
> Colbert
> Sent: Thursday, March 13, 2014 6:30 AM
> To: Sharon Scalise; histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Cornflaking artifact
> You will also see the cornflaking if your tissue is lifting off of the
> slide at all.  We used to get this more often on hard, decal specimens
> than on other specimens.  We used the film to coverslip.  If you remove
> the film from the problem slides and recoverslip conventionally with 
extra
> mountant and glass coverslips, I'm sure you will not see the artifact.
> Laurie Colbert
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [
> mailto:histonet-bounces <@t> lists.utsouthwestern.edu
<histonet-bounces <@t> lists.utsouthwestern.edu>]
> On Behalf Of Sharon
> Scalise
> Sent: Wednesday, March 12, 2014 8:00 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Cornflaking artifact
> I am looking for help with "cornflaking" (tiny, brown dry spots under
> coverslip)artifact.  We have been using fresh xylene on our stainer and
> coverslipper, cleaned and wiped all containers dry before filling, tried
> different lots of coverslipping film and had service on our coverslipper
> to make sure it was functioning properly, including the xylene drip.  We
> continue to have this artifact and it is driving us crazy.  It is 
sporadic
> with no pattern of tissue type or placement on the slide.  Sometimes it
> lands on tissue other times not.  Most of the time when we remove the
> coverslip and re-coverslip it goes away (I am assuming because the 
acetone
> removes any minute amounts of water that may be present).  We just 
cannot
> figure out where the water is coming from.  Has anyone seen this 
artifact
> while using the drying step on the prisma stainer?  We just recently
> started using the drying on some slides and I am thinking maybe it is
> causing humidity???  I cannot say for a fact that our "cornflaking"
> started at the same time, but it is suspicious. HELP!!!!!
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
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