[Histonet] RE: Eosin Leaching

Mon Jun 23 13:03:22 CDT 2014

Have you changed your reagent alcohol or has the company you buy from changed where it purchases the reagent alcohol?  Reagent alcohols with higher volumes of isopropanol than  5% can cause the eosin to leach out.  It will leave a green powder in the bottom of you staining dish.  Methanol and Isopropanol should be 5% each with 90% ethanol for the safest blend and what most of us old folks always thought reagent alcohol would stay.  We were wrong and different companied can change vendors or sources and get something they were not expecting too.   If you look up reagent alcohol or SDA reagent alcohol you find it has many formulas and the only real requirement by the ATF is to make the alcohol "non-drinkable or poison to humans".  I have seen formulas with 5 to 8% methanol and isopropanol with as low as 87% ethanol.  The percentage will depend on price as they have no idea at the manufacturing level what we use ti for or why.

Pam Marcum
UAMS

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amos, Lori (VDH)
Sent: Monday, June 23, 2014 12:29 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Eosin Leaching

I too have been experiencing Eosin leaching. I have tried all different options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols and xylenes that follow, even added a running water wash step after the Eosin ( to remove excess). Before this, I have had no problems and had made no changes to my daily activities. I rotate solutions daily. Aside from changing the company I buy my Eosin from, I am at a loss.
Thanks in advance for your help.
Lori

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, June 23, 2014 1:08 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 127, Issue 28

Send Histonet mailing list submissions to
	histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
	histonet-request <@t> lists.utsouthwestern.edu

You can reach the person managing the list at
	histonet-owner <@t> lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."

Today's Topics:

   1. RE: Eosin Leaching (Tony Reilly)
   2. Histology Benchmarking Survey (Lesley Bechtold)

----------------------------------------------------------------------

Message: 1
Date: Mon, 23 Jun 2014 09:22:28 +1000
From: Tony Reilly <Tony.Reilly <@t> health.qld.gov.au>
Subject: RE: [Histonet] Eosin Leaching
To: "Rathborne, Toni" <Toni.Rathborne <@t> rwjuh.edu>,	"Sanders, Jeanine
	(CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>, "'Adrienne 	Anderson'"
	<rennie1108 <@t> yahoo.com>, "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<E8088C46632C1C489B85680DD5C0D76B028140C8AD35 <@t> EXCCMSP10.qh.health.qld.gov.au>

Content-Type: text/plain; charset="iso-8859-1"

Hi Adrienne

Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin.

Regards
Tony

Tony Reilly B.App.Sc,  M.Sc
Chief Scientist
Anatomical Pathology
Pathology Queensland PAH
_________________________________________________
Health Services Support Agency| Department of Health

Building 15, Level 1,?
199 Ipswich Road?
WOOLLOONGABBA? Queensland 4102
Ph: 07 3176 2412
Mob: 0402139411
Fax: 07 3176 2930
Email: tony.reillyi <@t> health.qld.gov.au | www.health.qld.gov.au ? ? ?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, 20 June 2014 10:11 PM
To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histonet <@t> listsutsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching

Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID)
Sent: Thursday, June 19, 2014 5:26 PM
To: 'Adrienne Anderson'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching

Have you changed your coverslipping mountant?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson
Sent: Thursday, June 19, 2014 5:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem?

Thanks in advance for your help!

Adrienne

Adrienne Anderson, BS, HTL(ASCP)
Histotechnologist
Phylogeny, Inc. 
1476 Manning Pkwy, Powell, Ohio 43605
Phone: (614) 846-6161 
Fax:  (877) 591-1815

This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law.
Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. 
If you are not the intended recipient, please notify the sender and delete this message from your system. 
Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

********************************************************************************
This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error.
Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited.  The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters.
If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email.  You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced.
If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited.
Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email.
Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government.
**********************************************************************************

------------------------------

Message: 2
Date: Mon, 23 Jun 2014 15:25:27 +0000
From: Lesley Bechtold <Lesley.Bechtold <@t> jax.org>
Subject: [Histonet] Histology Benchmarking Survey
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<08997064E2075247A4DD5A035DB51CDF64FA0094 <@t> jaxbhexms02.jax.org>
Content-Type: text/plain; charset="us-ascii"

Dear Histonetters,

We are seeking information from our peers in the Histology world.  To better understand what other Histology Labs offer, we have created a short survey that we hope you will fill out. The link to the survey is below.

Information is a useful tool when preparing budgets, hiring staff, buying equipment or making the decision to add new processes to your lab.  Anyone who fills out the survey will receive a copy of the de-identified results.

We thank you in advance for participating.

If surveys annoy you, please delete this email.

Thank you.

Lesley

Lesley S. Bechtold
Senior Manager, Histopathology Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322 (phone)
207-288-6325 (fax)

https://www.surveymonkey.com/s/HistoSurvey2014

The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible.

------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 127, Issue 28
*****************************************

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

----------------------------------------------------------------------
Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.