[Histonet] RE: Eosin Leaching

Mon Jun 23 12:29:24 CDT 2014

I too have been experiencing Eosin leaching. I have tried all different options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols and xylenes that follow, even added a running water wash step after the Eosin ( to remove excess). Before this, I have had no problems and had made no changes to my daily activities. I rotate solutions daily. Aside from changing the company I buy my Eosin from, I am at a loss.
Thanks in advance for your help.
Lori

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Today's Topics:

   1. RE: Eosin Leaching (Tony Reilly)
   2. Histology Benchmarking Survey (Lesley Bechtold)

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Message: 1
Date: Mon, 23 Jun 2014 09:22:28 +1000
From: Tony Reilly <Tony.Reilly <@t> health.qld.gov.au>
Subject: RE: [Histonet] Eosin Leaching
To: "Rathborne, Toni" <Toni.Rathborne <@t> rwjuh.edu>,	"Sanders, Jeanine
	(CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>, "'Adrienne 	Anderson'"
	<rennie1108 <@t> yahoo.com>, "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
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	<E8088C46632C1C489B85680DD5C0D76B028140C8AD35 <@t> EXCCMSP10.qh.health.qld.gov.au>

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Hi Adrienne

Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin.

Regards
Tony

Tony Reilly B.App.Sc,  M.Sc
Chief Scientist
Anatomical Pathology
Pathology Queensland PAH
_________________________________________________
Health Services Support Agency| Department of Health

Building 15, Level 1,?
199 Ipswich Road?
WOOLLOONGABBA? Queensland 4102
Ph: 07 3176 2412
Mob: 0402139411
Fax: 07 3176 2930
Email: tony.reillyi <@t> health.qld.gov.au | www.health.qld.gov.au ? ? ?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, 20 June 2014 10:11 PM
To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histonet <@t> listsutsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching

Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID)
Sent: Thursday, June 19, 2014 5:26 PM
To: 'Adrienne Anderson'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching

Have you changed your coverslipping mountant?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson
Sent: Thursday, June 19, 2014 5:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem?

Thanks in advance for your help!

Adrienne

Adrienne Anderson, BS, HTL(ASCP)
Histotechnologist
Phylogeny, Inc. 
1476 Manning Pkwy, Powell, Ohio 43605
Phone: (614) 846-6161 
Fax:  (877) 591-1815

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Message: 2
Date: Mon, 23 Jun 2014 15:25:27 +0000
From: Lesley Bechtold <Lesley.Bechtold <@t> jax.org>
Subject: [Histonet] Histology Benchmarking Survey
To: "histonet <@t> lists.utsouthwestern.edu"
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Dear Histonetters,

We are seeking information from our peers in the Histology world.  To better understand what other Histology Labs offer, we have created a short survey that we hope you will fill out. The link to the survey is below.

Information is a useful tool when preparing budgets, hiring staff, buying equipment or making the decision to add new processes to your lab.  Anyone who fills out the survey will receive a copy of the de-identified results.

We thank you in advance for participating.

If surveys annoy you, please delete this email.

Thank you.

Lesley

Lesley S. Bechtold
Senior Manager, Histopathology Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322 (phone)
207-288-6325 (fax)

https://www.surveymonkey.com/s/HistoSurvey2014

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