[Histonet] Eosin Leaching

[Tony Reilly]

Hi Adrienne

Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin.

Regards
Tony


Tony Reilly B.App.Sc,  M.Sc
Chief Scientist
Anatomical Pathology
Pathology Queensland PAH
_________________________________________________
Health Services Support Agency| Department of Health

Building 15, Level 1, 
199 Ipswich Road 
WOOLLOONGABBA  Queensland 4102
Ph: 07 3176 2412
Mob: 0402139411
Fax: 07 3176 2930
Email: tony.reillyi <@t> health.qld.gov.au | www.health.qld.gov.au 
     





-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, 20 June 2014 10:11 PM
To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histonet <@t> listsutsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching

Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID)
Sent: Thursday, June 19, 2014 5:26 PM
To: 'Adrienne Anderson'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching

Have you changed your coverslipping mountant?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson
Sent: Thursday, June 19, 2014 5:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem?

Thanks in advance for your help!

Adrienne



Adrienne Anderson, BS, HTL(ASCP)
Histotechnologist
Phylogeny, Inc. 
1476 Manning Pkwy, Powell, Ohio 43605
Phone: (614) 846-6161 
Fax:  (877) 591-1815

This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law.
Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. 
If you are not the intended recipient, please notify the sender and delete this message from your system. 
Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

********************************************************************************
This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error.
Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited.  The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters.
If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email.  You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced.
If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited.
Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email.
Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government.
**********************************************************************************