[Histonet] Eosin Leaching

[Vanessa Avalos]

We had the same problem. Increase your Alcohol/Reagents (following Eosin) times as well as using fresh ones. I use 80% for about 20 sec and (2)100% for a minute each and (3) Xylene Subs for 2 min each. I also use a fresh 80% and rotate the 100% each day. If using a Xylene Sub it could also be a possibility that your mounting medium is not compatible. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson
Sent: Thursday, June 19, 2014 2:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem?

Thanks in advance for your help!

Adrienne



Adrienne Anderson, BS, HTL(ASCP)
Histotechnologist
Phylogeny, Inc. 
1476 Manning Pkwy, Powell, Ohio 43605
Phone: (614) 846-6161 
Fax:  (877) 591-1815

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