[Histonet] Eosin Leaching

[Anita Buchiane]

I'd advise checking your dehydration alcohols with a hydrometer.  This "bleeding" happened to me once and it turned out the alcohols after the Eosin were not graded.  My lab aide accidently used 100% only instead of 70%, 95% and then 100%.  
Anita

Anita

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson
Sent: Friday, June 20, 2014 8:15 AM
To: Rathborne, Toni
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin Leaching

We haven't changed our mounting medium, but I'll have to check on the alcohol levels. When I stain (we hand stain only - very small lab), I always make sure the alcohol is higher than the eosin, but I have a lab assistant now, and it's very possible I didn't make that a point. I'll try that.

Any more tips are welcome still - thank you all!

Adrienne


On Jun 20, 2014, at 8:11 AM, Rathborne, Toni <Toni.Rathborne <@t> rwjuh.edu> wrote:

> Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem.
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID)
> Sent: Thursday, June 19, 2014 5:26 PM
> To: 'Adrienne Anderson'; 'histonet <@t> lists.utsouthwestern.edu'
> Subject: RE: [Histonet] Eosin Leaching
> 
> Have you changed your coverslipping mountant?
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson
> Sent: Thursday, June 19, 2014 5:24 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Eosin Leaching
> 
> Hello all,
> 
> We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem?
> 
> Thanks in advance for your help!
> 
> Adrienne
> 
> 
> 
> Adrienne Anderson, BS, HTL(ASCP)
> Histotechnologist
> Phylogeny, Inc. 
> 1476 Manning Pkwy, Powell, Ohio 43605
> Phone: (614) 846-6161 
> Fax:  (877) 591-1815
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