[Histonet] Eosin Leaching

[Michael LaFriniere]

I have seen this happen with high levels of Isopropanol in alcohols after the eosins, I switch to regular reagent alcohols  and seemed to eliminate the bleeding of the eosin.

Michael
Michael R. LaFriniere, HT (ASCP) 
Executive Director
 

Capital Choice Pathology Laboratory
12041 Bournefield Way, Suite A * Silver Spring, MD 20904  
P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844
michael.lafriniere <@t> CCPLab.com
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson
Sent: Thursday, June 19, 2014 5:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem?

Thanks in advance for your help!

Adrienne



Adrienne Anderson, BS, HTL(ASCP)
Histotechnologist
Phylogeny, Inc. 
1476 Manning Pkwy, Powell, Ohio 43605
Phone: (614) 846-6161 
Fax:  (877) 591-1815

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