[Histonet] Processor malfunction - tissues not submerged ~ 9 hours

jsjurczak <@t> comcast.net jsjurczak <@t> comcast.net
Fri Jun 6 13:43:13 CDT 2014


Yet another reason to go microwave. This can't happen. 

----- Original Message -----

From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu> 
To: "Bill O'Donnell" <billodonnell <@t> catholichealth.net>, "Carla Conway" <cmconway <@t> usgs.gov>, histonet <@t> lists.utsouthwestern.edu 
Sent: Friday, June 6, 2014 12:53:07 PM 
Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours 

Oh where is the like button. :) 


-----Original Message----- 
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill 
Sent: Friday, June 06, 2014 12:22 PM 
To: Conway, Carla; histonet <@t> lists.utsouthwestern.edu 
Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours 

Not since 1978. (Sorry - couldn't resist - Happy Friday!) 

-----Original Message----- 
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Conway, Carla 
Sent: Friday, June 06, 2014 9:15 AM 
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours 

Hello everyone, 

When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect? 

Thanks very much, 

Carla 





Carla Conway 
Histology Technician 
Western Fisheries Research Center, USGS 
6505 N.E. 65th Street 
Seattle, WA 98115-5016 USA 
Phone: 206-526-2042 
Fax: 206-526-6654 
E-mail: cmconway <@t> usgs.gov 
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