[Histonet] Processor malfunction - tissues not submerged ~ 9 hours

[Shirley A. Powell]

Oh where is the like button.  :)


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 06, 2014 12:22 PM
To: Conway, Carla; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Not since 1978. (Sorry - couldn't resist - Happy Friday!)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Friday, June 06, 2014 9:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Hello everyone,

When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect?

Thanks very much,

Carla





Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmconway <@t> usgs.gov
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