[Histonet] microglia antibodies

[Kiousis, Sam]

I have been trying to get anitbodies for microglia (CD68, CD11b, IBA-1) to work using IHC and IF (regular, co-localization, even TSA amplification) on slides from frozen tissue that has been fixed in PFA and embedded in OCT with mixed crappy results.  Does anyone have any suggestions or protocols?

Thanks in advance

Sam Kiousis
Karmanos Cancer Institute
skiousis <@t> med.wayne.edu<mailto:skiousis <@t> med.wayne.edu>