[Histonet] RE: microglia antibodies

[Elizabeth Chlipala]

What species are you looking to stain?

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kiousis, Sam
Sent: Wednesday, June 04, 2014 12:53 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] microglia antibodies


I have been trying to get anitbodies for microglia (CD68, CD11b, IBA-1) to work using IHC and IF (regular, co-localization, even TSA amplification) on slides from frozen tissue that has been fixed in PFA and embedded in OCT with mixed crappy results.  Does anyone have any suggestions or protocols?

Thanks in advance

Sam Kiousis
Karmanos Cancer Institute
skiousis <@t> med.wayne.edu<mailto:skiousis <@t> med.wayne.edu>

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