[Histonet] Is antigen retrieval open on the Leica BOND RX IHC

[Wesley C. Minto]

Hey Jamie,

Thanks for the feedback. That is very useful to know. I was hoping that an
open container in the reagent rack might be used for AR, but it sounds like
we are limited to the bulk containers. This might make the difference in
choosing the Discovery Ultra over the Bond RX. Thanks again.

Wes


On Tue, Jun 3, 2014 at 10:43 AM, Erickson, Jamie E <
jamie.erickson <@t> abbvie.com> wrote:

> Hi Wes,
>                    We have 3 bond RX units and use them for animal tissue
> staining and we try all kinds of reagents (ab, chromogens, counterstains,
> acid washes) . As far as the HEIR and Enzyme go Leica has their own HEIR
> (citrate & EDTA) and an Enzyme (PTK). You can run a Dako PTK which I do at
> room temp or at 37C but as far as the HEIR you are limited to their Citrate
> Ph= 6 and EDTA Ph=9, I think I'm right on the Ph's but not sure.
> Anyway, we have found that all of our Dako protocols using PT modules or
> Pressure cooker  transferred  nicely to Leica and there recommended times
> for citrate and EDTA are pretty  darn good.  It is a learning curve and
> there are Leica ways of doing things but you can  get  to do almost
> anything you can do on the bench. We are working up a lot of double now and
> there are so many ways to do it.
> Hope that helps.
>
> Jamie
>
> JAMIE ERICKSON
> Scientist II
> HTL (ASCP)CM,MS
>
>
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-- 
Wesley C. Minto
Research & Drug Discovery
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