[Histonet] Is antigen retrieval open on the Leica BOND RX IHC

[Erickson, Jamie E]

Hi Wes,
                   We have 3 bond RX units and use them for animal tissue staining and we try all kinds of reagents (ab, chromogens, counterstains, acid washes) . As far as the HEIR and Enzyme go Leica has their own HEIR (citrate & EDTA) and an Enzyme (PTK). You can run a Dako PTK which I do at room temp or at 37C but as far as the HEIR you are limited to their Citrate Ph= 6 and EDTA Ph=9, I think I'm right on the Ph's but not sure. 
Anyway, we have found that all of our Dako protocols using PT modules or Pressure cooker  transferred  nicely to Leica and there recommended times for citrate and EDTA are pretty  darn good.  It is a learning curve and there are Leica ways of doing things but you can  get  to do almost anything you can do on the bench. We are working up a lot of double now and there are so many ways to do it.
Hope that helps.

Jamie

JAMIE ERICKSON
Scientist II
HTL (ASCP)CM,MS


Abbvie Bioreseach Center
Pharmacology
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Worcester,Ma 01605
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