[Histonet] RE: Cytokeratin AE1/AE3

[Cartun, Richard]

"Extra-follicular reticulm cells" contain cytokeratin and will label with antibodies to low molecular weight cytokeratin.  Could that be what you are seeing?

Reference:  Linden MD and Zarbo RJ:  Cytokeratin immunostaining patterns of benign, reactive lymph nodes:  applications for the evaluation of sentinel lymph node specimen.  Appl Immunohistochem Mol Morphol 2001;9(4):297-301.

On another note,  the recommendation now is to not do CK IHC on sentinel lymph nodes (except for rare cases).  Lymph node involvement should be based on H&E; not IHC.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 Fax
richard.cartun <@t> hhchealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy
Sent: Thursday, July 10, 2014 12:08 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Cytokeratin AE1/AE3

Hi Histonetters,

I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells".
We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining.
I was wondering if anyone else has come across this, and if they have fixed it?

Thank you,

Brandy Burnett, HTL
Cape Cod Hospital

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