[Histonet] RE: Cytokeratin AE1/AE3

[Barbara Tibbs]

What are you using for HIER?  Citrate buffer?  What pH?  
I use a low pH citrate buffer at 95C for 20 minutes and get good results.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.tibbs <@t> accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508


________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu <histonet-bounces <@t> lists.utsouthwestern.edu> on behalf of Burnett, Brandy <bburnett <@t> CapeCodHealth.org>
Sent: Thursday, July 10, 2014 3:08 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Cytokeratin AE1/AE3

Hi Histonetters,

I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our
New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells".
We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining.
I was wondering if anyone else has come across this, and if they have fixed it?

Thank you,

Brandy Burnett, HTL
Cape Cod Hospital

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