[Histonet] RE: Cytokeratin AE1/AE3

[Rathborne, Toni]

Would you share the source for the  sentinel lymph node recommendation mentioned? Although I just did a quick search, I didn't see anything on the CAP website.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Thursday, July 10, 2014 4:08 PM
To: Burnett, Brandy; 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: Cytokeratin AE1/AE3

"Extra-follicular reticulm cells" contain cytokeratin and will label with antibodies to low molecular weight cytokeratin.  Could that be what you are seeing?

Reference:  Linden MD and Zarbo RJ:  Cytokeratin immunostaining patterns of benign, reactive lymph nodes:  applications for the evaluation of sentinel lymph node specimen.  Appl Immunohistochem Mol Morphol 2001;9(4):297-301.

On another note,  the recommendation now is to not do CK IHC on sentinel lymph nodes (except for rare cases).  Lymph node involvement should be based on H&E; not IHC.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 Fax
richard.cartun <@t> hhchealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy
Sent: Thursday, July 10, 2014 12:08 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Cytokeratin AE1/AE3

Hi Histonetters,

I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells".
We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining.
I was wondering if anyone else has come across this, and if they have fixed it?

Thank you,

Brandy Burnett, HTL
Cape Cod Hospital

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error  contact the Help Desk for Cape Cod Healthcare.

Helpdesk <@t> CapeCodHealth.org
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet