[Histonet] Cytokeratin AE1/AE3

Burnett, Brandy bburnett <@t> CapeCodHealth.org
Thu Jul 10 11:08:09 CDT 2014

Hi Histonetters,

I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our
New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells".
We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining.
I was wondering if anyone else has come across this, and if they have fixed it?

Thank you,

Brandy Burnett, HTL
Cape Cod Hospital

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