[Histonet] marking tiny specimens
Barbara Tibbs
barbara.tibbs <@t> accuratediagnosticlabs.com
Fri Jan 31 11:53:02 CST 2014
Eyelashes are pointed at the end. Other body hair is not. I would re-use my tiny "paintbrush" over and over. No need to pull out an eyelash every day!
Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.tibbs <@t> accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508
________________________________
From: John Kiernan <jkiernan <@t> uwo.ca>
Sent: Friday, January 31, 2014 4:21 AM
To: Barbara Tibbs; Cheryl Crowder; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] marking tiny specimens
Dedication to duty! Ouch! Did you ever run out of eyelashes? Why not a short eyebrow or forearm hair?
JK
= = =
On 30/01/14, Barbara Tibbs <barbara.tibbs <@t> accuratediagnosticlabs.com> wrote:
Are you mixing Eosin in the last two alcohols of your processor? That should work. If you are dotting the specimens with Eosin before placing in a cassette, the dye will wash out early in the processing.
Also, I don't know if this helps you but when I had to ink mouse arteries I would glue one of my eyelashes to the end of a wood applicator stick and use the eyelash as a tiny paint brush dipped in India ink. I would do this under a dissecting microscope. Maybe you could do that with the eggs?
Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.tibbs <@t> accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu <histonet-bounces <@t> lists.utsouthwestern.edu> on behalf of Cheryl Crowder <ccrowder <@t> vetmed.lsu.edu>
Sent: Thursday, January 30, 2014 5:59 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] marking tiny specimens
I am processing some extremely small specimens - pin tip size. These are
eggs with a protein covering. I have tried using eosin to color the tissues
before processing but the color came out before paraffin. The coating on
the eggs will not absorb the dye. Does anyone have a suggestion for dyeing
or marking these tissues so I can see them better to embed. Thanks in
advance,
Cheryl
Cheryl Crowder, BA, HTL(ASCP)
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