[Histonet] marking tiny specimens

[John Kiernan]

Dedication to duty! Ouch! Did you ever run out of eyelashes?  Why not a short eyebrow or forearm hair?
JK
= = = 
On 30/01/14, Barbara Tibbs <barbara.tibbs <@t> accuratediagnosticlabs.com> wrote: 
>  Are you mixing Eosin in the last two alcohols of your processor?   That should work.  If you are dotting the specimens with Eosin before placing in a cassette, the dye will wash out early in the processing.
> 
> Also, I don't know if this helps you but when I had to ink mouse arteries I would glue one of my eyelashes to the end of a wood applicator stick and use the eyelash as a tiny paint brush dipped in India ink.  I would do this under a dissecting microscope.  Maybe you could do that with the eggs?
> 
> Barbara S. Tibbs
> Histology Supervisor
> Accurate Diagnostic Labs
> South Plainfield, NJ
> barbara.tibbs <@t> accuratediagnosticlabs.com
> 732-839-3374
> Cell: 610-809-6508
> 
> 
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu <histonet-bounces <@t> lists.utsouthwestern.edu> on behalf of Cheryl Crowder <ccrowder <@t> vetmed.lsu.edu>
> Sent: Thursday, January 30, 2014 5:59 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] marking tiny specimens
> 
> I am processing some extremely small specimens - pin tip size.  These are
> eggs with a protein covering.  I have tried using eosin to color the tissues
> before processing but the color came out before paraffin.  The coating on
> the eggs will not absorb the dye.  Does anyone have a suggestion for dyeing
> or marking these tissues so I can see them better to embed.  Thanks in
> advance,
> Cheryl
> 
> Cheryl Crowder, BA, HTL(ASCP)
> 
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