[Histonet] Cell Marque Mammoglobin (31A5) New Protocol?

Abby L. Maples mrs.abby.maples <@t> gmail.com
Wed Jan 22 11:02:25 CST 2014


Thank you everyone! You've all been so helpful regarding the mammaglobin.
Ventana has called me and I think we got things figured out (at least
tenatively) and the wheels are in motion. If it still doesn't work, our rep
will come out and see us. Thanks again for your input! Great ideas abound!!


On Wed, Jan 22, 2014 at 10:44 AM, Thomas Jasper <Thomas.Jasper <@t> deaconess.com
> wrote:

> Hi Abby,
>
> I have a couple of suggestions -
>
> 1) Try to extend your incubation times.  You may need to do a couple-three
> of these to get a decent result.
>
> 2) Contact Ventana and have a field specialist come out and help you with
> working out this problem on the Benchmark.  My experiences with Ventana
> have been positive in this regard and I've always found them willing to
> help in any way possible.
>
> You may have already thought of, and done these things, I don't know.
>  Just thought I'd mention them.
>
> Good luck to you,
> Tom Jasper
>
>
> Thomas Jasper HT (ASCP) BAS
> AP/CP Supervisor
> Deaconess Hospital
> 600 Mary Street
> Evansville, IN 47747
> Thomas.jasper <@t> deaconess.com
> 812-450-2485
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Abby L. Maples
> Sent: Wednesday, January 22, 2014 8:11 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol?
>
> Fellow histotechs,
>
> We've been running mammoglobin testing on our Ventana Benchmark XT.
> However, Cell Marque (our supplier of mammoglobin antibody) has changed
> the concentration. Our old concentration was 0.12 mg/mL, the new
> concentration is weaker at 0.04 mg/mL. The antibody is a rabbit monoclonal.
> We tried running the old protocol with the new concentration, but the
> results were not adequate (see below). We tried running the protocol given
> to us by Cell Marque, but theirs is specific towards the Benchmark
> UltraView and also returned inadequate results.
>
> We process our tissue from formalin to paraffin. Our lab uses the iVew DAB
> detection kit.
>
> Our old protocol:
> Deparaffinization
> Conditioner #1 (short 8 minute conditioning) Mild CC1 (mild 30 minutes
> conditioning) Standard CC1 (standard 60 minute conditioning) No enzyme 37C
> antibody incubation temperature No titration
> Antibody: Mammoglobin 31A5 at 0.12 mg/mL concentration for 32 minutes A/B
> Block Hematoxylin counterstain 4 minutes Blueing post counterstain 4 minutes
>
> Does anyone else use this product from Ventana/Cell Marque? Does anyone
> have a working protocol for a weaker antibody titration?
>
> Any help would be greatly appreciated!
>
> *Abby L. H. Maples, BS, MPH, HTL (ASCP)CM* Histotechnologist, Surgical
> Pathology Mercy Medical Center
> 701 10th Street SE
> Cedar Rapids, IA 52403
> Lab Phone: (319) 398-6827
> Cell: (319) 432-1530
> Fax: (319) 221-8767
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>


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