[Histonet] RE: Zebra-fish embryo histology

[Bonnie Gambichler]

Patty,

Our lab has processed zebra fish embryos for paraffin sectioning.  The embryos were fixed overnight in 4% paraformaldehyde and processed on a biopsy program without any heat.  The key is to pop the swim bladders of the fish so that they will lay flat during embedding.  We put multiple pieces into each biopsy sized mold and were able to get a good section with intestine on at least one of the fish in each block.  It is imperative that you soak the blocks, on a paper towel moistened with diluted ammonium hydroxide, on ice for at least an hour to get decent sections because the tissue is very dry.

Best Regards,

Bonnie Gambichler, HT ( ASCP )
JHU Oncology Tissue Services Lab