[Histonet] Cell Marque Mammoglobin (31A5) New Protocol?

[Mike Thompson]

Diagnostic Biosystems has Rabbit Monos

Website below

Mike

Michael O. Thompson 
Director of Sales
Diagnostic BioSystems
Phone: 1-888-896-3350
Mobile: 412-860-1288
Office Fax: 412-727-6080

"IHC Made Affordable"
 www.dbiosys.com

joelle weaver <joelleweaver <@t> hotmail.com> wrote:

>also have IVD mammoglobin, clone  31A5 rabbit monoclonal, works really well for me at 1:75-  if you need/prefer IVD from cancer diagnostics, the other I posted is ASR. 
>
>
>
>
>Joelle Weaver MAOM, HTL (ASCP) QIHC
> 
>> From: mrs.abby.maples <@t> gmail.com
>> Date: Wed, 22 Jan 2014 08:11:11 -0600
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol?
>> 
>> Fellow histotechs,
>> 
>> We've been running mammoglobin testing on our Ventana Benchmark XT.
>> However, Cell Marque (our supplier of mammoglobin antibody) has changed the
>> concentration. Our old concentration was 0.12 mg/mL, the new concentration
>> is weaker at 0.04 mg/mL. The antibody is a rabbit monoclonal. We tried
>> running the old protocol with the new concentration, but the results were
>> not adequate (see below). We tried running the protocol given to us by Cell
>> Marque, but theirs is specific towards the Benchmark UltraView and also
>> returned inadequate results.
>> 
>> We process our tissue from formalin to paraffin. Our lab uses the iVew DAB
>> detection kit.
>> 
>> Our old protocol:
>> Deparaffinization
>> Conditioner #1 (short 8 minute conditioning)
>> Mild CC1 (mild 30 minutes conditioning)
>> Standard CC1 (standard 60 minute conditioning)
>> No enzyme
>> 37C antibody incubation temperature
>> No titration
>> Antibody: Mammoglobin 31A5 at 0.12 mg/mL concentration for 32 minutes
>> A/B Block
>> Hematoxylin counterstain 4 minutes
>> Blueing post counterstain 4 minutes
>> 
>> Does anyone else use this product from Ventana/Cell Marque? Does anyone
>> have a working protocol for a weaker antibody titration?
>> 
>> Any help would be greatly appreciated!
>> 
>> *Abby L. H. Maples, BS, MPH, HTL (ASCP)CM*
>> Histotechnologist, Surgical Pathology
>> Mercy Medical Center
>> 701 10th Street SE
>> Cedar Rapids, IA 52403
>> Lab Phone: (319) 398-6827
>> Cell: (319) 432-1530
>> Fax: (319) 221-8767
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