[Histonet] Warthin Starry Help

[Roberta Horner]

My hydroquonine was received in the lab in 1980 and it works.  As long as my control is positive I am allowed to keep it but then I work in a veterinary diagnostic lab and am not under all the regulations that those of you in hospitals are under.
Roberta Horner HT/HTL
Animal Diagnostic Lab
Penn State University

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald
Sent: Tuesday, January 14, 2014 10:16 AM
To: Campbell, Tasha M.
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Warthin Starry Help


Hydroquinone has a short shelf life once opened.

> On Jan 14, 2014, at 6:59 AM, "Campbell, Tasha M." <tmcampbell <@t> fmh.org>
wrote:
>
> I need help with warthin starry stain please.  I am a very small lab 
> trying to save money so I want to make the reagents myself.  I have 
> sigma Aldrich dry reagents and have been making the reagents and 
> following the instructions correctly and I did get the stain to work 
> wonderfully when I first started.  Then suddenly it quit working and I 
> cannot get it to work again.  I have made fresh reagents day after day 
> and I have a pH of 3.8 for the acidulated water.  The tissue will 
> stain normally with the yellow to brown background and I the 
> developing solution turns black when it is left on paper but the 
> h.pylori organisms are not staining.  I am getting very frustrated and 
> need some help please.
>
>
>
> Thank you.
>
>
>
> Tasha Campbell, HTL
>
> tmcampbell <@t> fmh.org
>
>
>
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