[Histonet] Warthin Starry Help

[Campbell, Tasha M.]

I was wondering about that.  Even in its crystal form?  I've been trying
to test every possible problem, though, and I am using a kit right now
so I have substituted the kit's hydroquinone in place of mine and used
the rest of my reagents.  It still didn't work. So this has to mean
there is another problem.  But with the entire kit's reagents it works.
I have narrowed down by substituting my reagents in for the kit's one by
one and the only combination I can get to work is using my 1% silver for
impregnation and then the kit's reagents for developing so this has to
mean that my silver is good.

 
 
 
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)

-----Original Message-----
From: Jennifer MacDonald [mailto:JMacDonald <@t> mtsac.edu] 
Sent: Tuesday, January 14, 2014 10:16 AM
To: Campbell, Tasha M.
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Warthin Starry Help


Hydroquinone has a short shelf life once opened.

> On Jan 14, 2014, at 6:59 AM, "Campbell, Tasha M." <tmcampbell <@t> fmh.org>
wrote:
>
> I need help with warthin starry stain please.  I am a very small lab
> trying to save money so I want to make the reagents myself.  I have
> sigma Aldrich dry reagents and have been making the reagents and
> following the instructions correctly and I did get the stain to work
> wonderfully when I first started.  Then suddenly it quit working and I
> cannot get it to work again.  I have made fresh reagents day after day
> and I have a pH of 3.8 for the acidulated water.  The tissue will
stain
> normally with the yellow to brown background and I the developing
> solution turns black when it is left on paper but the h.pylori
organisms
> are not staining.  I am getting very frustrated and need some help
> please.
>
>
>
> Thank you.
>
>
>
> Tasha Campbell, HTL
>
> tmcampbell <@t> fmh.org
>
>
>
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