[Histonet] Warthin Starry Help

[Jennifer MacDonald]

Hydroquinone has a short shelf life once opened.

> On Jan 14, 2014, at 6:59 AM, "Campbell, Tasha M." <tmcampbell <@t> fmh.org>
wrote:
>
> I need help with warthin starry stain please.  I am a very small lab
> trying to save money so I want to make the reagents myself.  I have
> sigma Aldrich dry reagents and have been making the reagents and
> following the instructions correctly and I did get the stain to work
> wonderfully when I first started.  Then suddenly it quit working and I
> cannot get it to work again.  I have made fresh reagents day after day
> and I have a pH of 3.8 for the acidulated water.  The tissue will stain
> normally with the yellow to brown background and I the developing
> solution turns black when it is left on paper but the h.pylori organisms
> are not staining.  I am getting very frustrated and need some help
> please.
>
>
>
> Thank you.
>
>
>
> Tasha Campbell, HTL
>
> tmcampbell <@t> fmh.org
>
>
>
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