[Histonet] Re: Fixed tissue storage ratio in 70% ethanol

Bob Richmond rsrichmond <@t> gmail.com
Fri Jan 3 13:06:26 CST 2014


Carla Conway at the Western Fisheries Research Center, in Seattle,
Washington state asks:

<<When transferring formalin-fixed tissues to 70% ethanol for storage, is
there a recommended ratio of ethanol volume to tissue volume?<<

Once the tissue is entirely fixed, it can be stored in the smallest volume
of 70% ethanol that will cover it. In my residency autopsy wet tissue
samples in 70% ethanol were stored in sealed plastic bags in a small volume
of it, and were intended to last for decades.

Bob Richmond
Samurai Pathologist
Maryville, Tennessee


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