[Histonet] Re: Fixed tissue storage ratio in 70% ethanol
Conway, Carla
cmconway <@t> usgs.gov
Fri Jan 3 16:27:52 CST 2014
Thank you, Bob!
Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-6282 ext. 242
Fax: 206-526-6654
E-mail: cmconway <@t> usgs.gov
On Fri, Jan 3, 2014 at 11:06 AM, Bob Richmond <rsrichmond <@t> gmail.com> wrote:
> Carla Conway at the Western Fisheries Research Center, in Seattle,
> Washington state asks:
>
> <<When transferring formalin-fixed tissues to 70% ethanol for storage, is
> there a recommended ratio of ethanol volume to tissue volume?<<
>
> Once the tissue is entirely fixed, it can be stored in the smallest volume
> of 70% ethanol that will cover it. In my residency autopsy wet tissue
> samples in 70% ethanol were stored in sealed plastic bags in a small volume
> of it, and were intended to last for decades.
>
> Bob Richmond
> Samurai Pathologist
> Maryville, Tennessee
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