[Histonet] beta gal antibody

[Mesru T]

Hi Elizabeth,

I always read and benefit from the messages here. Now I so happy that I
think I might be of some help to you or also others.

When I do enzymehistochemistry to detect the beta galactosidase enzimatic
activity using x-gal as a substrate, I mostly use fresh frozen tissues and
fix the frozen sections in 4% PFA + 0.2% gluteraldehyde in PBS for 5min.
When I do IHC I prefer to use paraffin sections (the morphology is superior
and I find it easier to cut paraffin than frozne).
Frozen sections wll work for IHC too. The enzyme does not need to be active
for antibody to bind during IHC satining.

I have tried many antibodies until I have found the one that works for me
on FFPE mouse tissues: Beta-galactosidase  from eBioscience cat#14-6773
lot#E05196-1631  I use it at 1ug/ml concentration.

I hope this helps.

Regards,
Mesruh Turkekul
mskcc.org