[Histonet] Vascular contrast agent injected tissue
abtdhu <@t> gmail.com
abtdhu <@t> gmail.com
Mon Aug 18 20:51:18 CDT 2014
Hi histoneters,
Does any one out there to process tissue in a tissue process machine after lead or barium agents introduced to your tissue? It will do any harm to the machine? Get clog or contaminate other tissue? Thanks for your help.
Dorothy Hu
MGH endocrine histocore
Sent from my iPad
> On Aug 18, 2014, at 1:00 PM, histonet-request <@t> lists.utsouthwestern.edu wrote:
>
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> Today's Topics:
>
> 1. RE: Tissue falling off (Susan.Walzer <@t> HCAHealthcare.com)
> 2. Technidata Synergy Histology/ Cytology module
> (Rogerson Kemlo (THE WALTON CENTRE NHS FOUNDATION TRUST - RET))
> 3. CD68 (Reuel Cornelia)
> 4. Floating Tissue Question (Gauch, Vicki)
> 5. RE: CD68 (Elizabeth Chlipala)
> 6. RE: Histonet Digest, Vol 129, Issue 25 (Algeo, Lacie A)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 18 Aug 2014 01:56:23 -0500
> From: <Susan.Walzer <@t> HCAHealthcare.com>
> Subject: [Histonet] RE: Tissue falling off
> To: <tbraud <@t> holyredeemer.com>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <4BF03F5404EBDE409AF9232DA74B9DED2FC11CA7E1 <@t> FWDCWPMSGCMS09.hca.corpad.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Tissue that has been properly processed, cut thin and dried well should not be falling off.. We have never needed any additive in our water baths but if we have tough bones or very bloody tissue we use plus slides. PS: we soak tough or bloody tissue in soapy water with some ammonia as it REALLY makes a difference.
>
> Susan Walzer HT(ASCP)
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Terri Braud
> Sent: Friday, August 15, 2014 1:52 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Tissue falling off
>
> Hi - Just a couple of suggestions -
> I've seen this periodically crop up during the years. There are a couple of things to consider:
> Water baths are completely clean - after dumpling water and removing paraffin debris with whatever you use - wipe out the bath with clean absolute alcohol.
> Be careful where you spray - make sure that if you use anything like Paraguard, or similar stuff that keeps paraffin from sticking, that it is used sparingly, or preferably, not at all. We had a situation where we had a trial sample that someone was using to clean her microtome and forceps and the stuff got everywhere and tissues started falling off.
> Your water in the waterbaths is clean - Fresh DI water. Once we had a contaminant in our DI water, and the patient tissues slid right off our slides. We use clean slides, good quality water and super clean waterbaths.
> Is your heater up and running? - Our slides are stained on a stainer with built in slide dryers. We used to have a problem with the first rack of patient slides' tissue falling off because the slide dryer was not hot enough for our short dry cycle. We now send a rack through first, which turns on the heaters, then load the patient rack.
> Hope this gives you a few ideas
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Holy Redeemer Hospital Laboratory
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> Ph: 215-938-3676
> Fax: 215-938-3874
>
> 7. tissue falling off slides (Burnett, Brandy)
> From: "Burnett, Brandy" <bburnett <@t> CapeCodHealth.org>
> Subject: [Histonet] tissue falling off slides
>
> Our lab has encountered an issue with tissue falling off of the slides.
> It is mostly the patient tissue
> that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much appreciated.
> Thanks Again,
> Brandy Burnett, HTL
>
>
> ---------------------------------------------------------------------------------
>
>
>
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> ------------------------------
>
> Message: 2
> Date: Mon, 18 Aug 2014 08:23:28 +0100
> From: "Rogerson Kemlo (THE WALTON CENTRE NHS FOUNDATION TRUST - RET)"
> <kemlo.rogerson1 <@t> nhs.net>
> Subject: [Histonet] Technidata Synergy Histology/ Cytology module
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <20140818072328.F1AC744840D <@t> nhs-pd1e-esg106.ad1.nhs.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Dear List,
>
> The Walton Centre is one of the few Neuropathology Departments in the UK to use TD Synergy Histo/ Cyto as its LIMS. It is also used in Neurobiochemistry.
>
> I am aware that this system is used in the US and wonder if anyone has any experience in its use?
>
> On the 'lighter side' I have been a HCPC registered Biomedical Scientist (UK's name for HistoTech) for 44 years as I started as a Junior Technician in March 1970 in Microbiology until I was exiled to Histopathology after a few years.
>
> Regards
>
> Kemlo Rogerson
> Pathology Systems Manager
> The Walton Centre
> Liverpool
>
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> ------------------------------
>
> Message: 3
> Date: Mon, 18 Aug 2014 09:27:22 -0500
> From: "Reuel Cornelia" <Reuel.Cornelia <@t> tsrh.org>
> Subject: [Histonet] CD68
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <53F1C6FA.0F7E.00C5.1 <@t> tsrh.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anybody out there knows a CD68 antibody that works with
> pig(Porcine) tissue. We have tried 3 CD68 antibodies already from Santa
> Cruz, Dako and Novus that they say works with pig tissue but it did not.
> We tried using heat antigen retrieval and enzyme digestion for paraffin,
> it did not work. We also use pig frozen tissue and still do not work.
> Any suggestion.
>
>
>
>
>
> Reuel Cornelia, BS MT, AMT
> Cellular Pathology
> Texas Scottish Rite Hospital for Children
> 2222 Welborn Street
> Dallas, TX 75219
> Tel: 214-559-7766
> fax: 214-559-7768
>
> ------------------------------
>
> Message: 4
> Date: Mon, 18 Aug 2014 15:06:16 +0000
> From: "Gauch, Vicki" <GauchV <@t> mail.amc.edu>
> Subject: [Histonet] Floating Tissue Question
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <BF796EC3274FE947B2FD0C2AD637CBE97AA8844F <@t> wexcmbx01v.AMCNT.AMC.EDU>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi everyone,
> I have been asked to post this question.... Our lab currently uses Cardinal Plus slides when cutting IHC and ER,PR,etc. on breast tissue. As of late, we are having issues with the tissue floating. It does not appear to be a fixation problem. Does anyone have any recommendations of other plus slide brands or tissue dryers,etc. that have worked well for them ? Please feel free to respond to my e-mail and I will pass the information on.
> Thank you for any help you can provide us,
> Vicki Gauch
> AMCH
> Albany, NY
>
>
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> ------------------------------
>
> Message: 5
> Date: Mon, 18 Aug 2014 10:08:11 -0600
> From: Elizabeth Chlipala <liz <@t> premierlab.com>
> Subject: RE: [Histonet] CD68
> To: Reuel Cornelia <Reuel.Cornelia <@t> tsrh.org>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <14E2C6176416974295479C64A11CB9AE019C79E021B3 <@t> SBS2K8.premierlab.local>
> Content-Type: text/plain; charset="us-ascii"
>
> We use MAC387 not exactly the same as CD68 but stains macrophages, monocytes and I think granulocytes.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 881-0763 cell
> (303) 682-9060 fax
> liz <@t> premierlab.com
>
> Ship to address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia [Reuel.Cornelia <@t> tsrh.org]
> Sent: Monday, August 18, 2014 8:27 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] CD68
>
> Does anybody out there knows a CD68 antibody that works with
> pig(Porcine) tissue. We have tried 3 CD68 antibodies already from Santa
> Cruz, Dako and Novus that they say works with pig tissue but it did not.
> We tried using heat antigen retrieval and enzyme digestion for paraffin,
> it did not work. We also use pig frozen tissue and still do not work.
> Any suggestion.
>
>
>
>
>
> Reuel Cornelia, BS MT, AMT
> Cellular Pathology
> Texas Scottish Rite Hospital for Children
> 2222 Welborn Street
> Dallas, TX 75219
> Tel: 214-559-7766
> fax: 214-559-7768
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 18 Aug 2014 16:18:18 +0000
> From: "Algeo, Lacie A" <Lacie.Algeo <@t> providence.org>
> Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 25
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <24C4B3C167E5694887AB594C7602CE3A0C2E64 <@t> WN35104.or.providence.org>
> Content-Type: text/plain; charset="us-ascii"
>
> I hope I am responding to the correct location :)
> Brandy....a few questions about your tissue falling off your slides. What kind of tissue? What kind of slides? What kind of stainer?
> Thanks,
> Lacie
>
> Lacie Algeo, HTL (ASCP) MBCM
> Histology Supervisor
> Providence Sacred Heart Medical Center Laboratory
> 101 W 8th Avenue
> L-2
> Spokane, WA 99204
> 509-474-4418
> FAX 509-474-2052
> lacie.algeo <@t> providence.org
>
>
> This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message.
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
> Sent: Saturday, August 16, 2014 10:09 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 129, Issue 25
>
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
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> When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. antibody suggestions CD8, FAP, Cd11b, CD11c (Mesru T)
> 2. RE: Tissue falling off (Terri Braud)
> 3. Re: RE: Glassware Cleaning again (darkdaym <@t> comcast.net)
> 4. Re: antibody suggestions CD8, FAP, Cd11b, CD11c (gayle callis)
> 5. Re: RE: Glassware Cleaning again (E. Wayne Johnson)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 15 Aug 2014 13:24:29 -0400
> From: Mesru T <turkekul <@t> gmail.com>
> Subject: [Histonet] antibody suggestions CD8, FAP, Cd11b, CD11c
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CACf3QnTPuLfJReDbsDqHcKPjGfiHfNJbdwAfcR-Pnzm4U8Vmcw <@t> mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Dear Histonetters,
>
>
> I need your help to find good antibodies for IHC on mouse and human FFPE tissues like normal tissues and tumors. Please share any information you think might be helpful.
>
> CD8 for IHC on mouse FFPE spleen or other lymphoid organs.
> FAP (Fibroblast Activated protein) for IHC on mouse and human FFPE tumors.
> CD11b for IHC on mouse FFPE spleen or other lymphoid organs..
> CD11c for IHC on mouse FFPE spleen or other lymphoid organs.
>
> Thanks in advance for all your help.
>
> Regards,
> Mesru
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 15 Aug 2014 13:52:09 -0400
> From: "Terri Braud" <tbraud <@t> holyredeemer.com>
> Subject: [Histonet] RE: Tissue falling off
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <BFBF7C6B9627B947B74CBEBD45CE368B14048B56 <@t> hrex-svr.holyredeemer.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi - Just a couple of suggestions -
> I've seen this periodically crop up during the years. There are a couple of things to consider:
> Water baths are completely clean - after dumpling water and removing paraffin debris with whatever you use - wipe out the bath with clean absolute alcohol.
> Be careful where you spray - make sure that if you use anything like Paraguard, or similar stuff that keeps paraffin from sticking, that it is used sparingly, or preferably, not at all. We had a situation where we had a trial sample that someone was using to clean her microtome and forceps and the stuff got everywhere and tissues started falling off.
> Your water in the waterbaths is clean - Fresh DI water. Once we had a contaminant in our DI water, and the patient tissues slid right off our slides. We use clean slides, good quality water and super clean waterbaths.
> Is your heater up and running? - Our slides are stained on a stainer with built in slide dryers. We used to have a problem with the first rack of patient slides' tissue falling off because the slide dryer was not hot enough for our short dry cycle. We now send a rack through first, which turns on the heaters, then load the patient rack.
> Hope this gives you a few ideas
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Holy Redeemer Hospital Laboratory
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> Ph: 215-938-3676
> Fax: 215-938-3874
>
> 7. tissue falling off slides (Burnett, Brandy)
> From: "Burnett, Brandy" <bburnett <@t> CapeCodHealth.org>
> Subject: [Histonet] tissue falling off slides
>
> Our lab has encountered an issue with tissue falling off of the slides.
> It is mostly the patient tissue
> that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much appreciated.
> Thanks Again,
> Brandy Burnett, HTL
>
>
> ---------------------------------------------------------------------------------
>
>
>
> CONFIDENTIALITY NOTICE:
>
> This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail.
> Please notify the sender of any error by E-Mail.
>
> Thank you for your cooperation.
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 15 Aug 2014 13:06:52 -0500
> From: darkdaym <@t> comcast.net
> Subject: Re: [Histonet] RE: Glassware Cleaning again
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <53EE4C3C.7070405 <@t> comcast.net>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Brian, et.al.,
> It's not a typo or and an error. It's one might call a variant spelling. The Merck Index lists Bromcresol Purple, CAS Number 115-40-2.
> Below is what Sigma lists with the same CAS number. I've noticed the prefix Brom or Bromo on the name of several dyes. Either way, it's the same thing.
>
> *114375 * *Sigma-Aldrich *
>
>
> Bromocresol Purple
>
>
> BioReagent, suitable for indicator, Dye content 90 %
>
> Synonym: *5,5?-Dibromo-/o/-cresolsulfonphthalein, Bromcresol purple sultone form
>
>
> *
>> On 8/15/2014 11:35 AM, Cooper, Brian wrote:
>> I noticed the discrepancy in spelling too. I looked online for like 30 minutes, and couldn't find anything called "Bromcresol." Found a lot of vendors selling Bromocresol Purple (and Green for that matter). Best I can figure is that this is a typo on CAP's checklist (that's been there for several years now).
>>
>> Thanks,
>>
>> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
>> Department of Pathology and Laboratory Medicine
>> Children's Hospital Los Angeles
>> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
>> bcooper <@t> chla.usc.edu
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
>> Sent: Friday, August 15, 2014 9:13 AM
>> To: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
>> Subject: [Histonet] Glassware Cleaning again
>>
>> Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple.
>>
>> Laurie Colbert, HT (ASCP)
>> Histology Supervisor
>> PATH MD
>> 8158 Beverly Blvd.
>> Los Angeles, CA 90048
>> (323) 648-3214 direct
>> (424) 245-7284 main lab
>>
>> The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message.
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>> ---------------------------------------------------------------------
>> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments,
>> is for the sole use of the intended recipient(s) and may contain confidential
>> or legally privileged information. Any unauthorized review, use, disclosure
>> or distribution is prohibited. If you are not the intended recipient, please
>> contact the sender by reply e-mail and destroy all copies of this original message.
>>
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>>
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>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 15 Aug 2014 15:10:27 -0600
> From: "gayle callis" <gayle.callis <@t> bresnan.net>
> Subject: [Histonet] Re: antibody suggestions CD8, FAP, Cd11b, CD11c
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000001cfb8cd$583fb3d0$08bf1b70$@bresnan.net>
> Content-Type: text/plain; charset="us-ascii"
>
> You Wrote:
>
>
>
> I need your help to find good antibodies for IHC on mouse and human FFPE
>
> tissues like normal tissues and tumors. Please share any information you
>
> think might be helpful.
>
>
>
> CD8 for IHC on mouse FFPE spleen or other lymphoid organs.
>
> FAP (Fibroblast Activated protein) for IHC on mouse and human FFPE tumors.
>
> CD11b for IHC on mouse FFPE spleen or other lymphoid organs..
>
> CD11c for IHC on mouse FFPE spleen or other lymphoid organs.
>
>
>
> Thanks in advance for all your help.
>
>
>
> Regards,
>
> Mesru
>
> ____________________________________________________________________________
> ___________________________________________
>
>
>
> I can answer some of the murine CD marker questions.
>
>
>
> CD8 on FFPE mouse tissues will never work. These markers are too strongly
> cross linked by the aldehyde based fixation, and PFA is not an alternative.
> You can try HIER and Enzyme digestions until you are blue in the face, and
> the staining will NOT work. This will also be true of CD11c dendritic cell
> Clone HL3, and CD11b Mac1.
>
>
>
> This means you will have to use fresh tissue frozen sections, acetone fixed
> for any staining success. We use an acetone/absolute ethanol mixture for
> fixation of murine tissue for all our CD marker staining, especially when
> you need to do all these CD markers on the same sample.
>
>
>
> As for positive tissue controls,
>
>
>
> CD8 Rat antiMouse monoclonal - normal spleen
>
> CD11c - Peyers patches from small intestine, lymph node and probably spleen
> will work.
>
> CD11b, Mac1 - we stimulated mouse to produce macrophages in Peyers patches
> located on small intestine. Stimulation was oral inoculation with an
> attenuated bacteria.
>
> FAP (Fibroblast Activated protein) for IHC on mouse and human FFPE tumors.
> Go to ABCAM, and look at cross reactivity for this polyclonal antibody. It
> works on FFPE mouse, human and two other species.
>
>
>
>
>
> I can't provide an answer for FFPE human tissues with any of these CD
> markers.
>
>
>
> I strongly suggest you go to BD Bioscience and do a search for the CD
> markers, especially the mouse that will be either rat and Armenian Hamster
> monoclonals. Read the Technical data sheets and see what applications will
> work or not in terms of IHC. We have used BD Bioscience, and eBioscience for
> our murine CD marker with great success but you have to be aware that CD8
> has more than one clone, some working better on solvent fixed murine tissues
> than others. This is also true of CD11c. ABCAM has an excellent website
> for these antibodies, but compare prices.
>
>
>
> There is another fixative that is formalin free, the Becksteads IHC zinc
> fixative and also PLP i.e. paraformaldehyde-lysine-Periodate. PLP may allow
> staining of these aldehyde compromised CD markers since the lysine helps get
> rid of free aldehydes, along with the periodate and is, however, a fixative
> that is bit tedious to use since it has to be made up fresh every time you
> use it. Beckstead originally developed IHC zinc fixative in order to do IHC
> on human tumors, lymphomas if I recall. I have original and other
> publications for both fixatives showing success with murine CD markers and
> other species. The IHC zinc fixative allows paraffin embedding and IHC
> staining of these markers, (also a publication by Nitta et al)
>
>
>
> Our lab used fresh tissue frozen sections, acetone/alcohol fixed exclusively
> for all CD marker IHC and/or single, double and triple immunofluroescence
> protocols.
>
>
>
> I will be happy to provide acetone/alcohol fixative method and alternative
> acetone fixations that work with murine CD markers. You cannot use
> acetone/alcohol for human CD8 or CD4 as the alcohol ruins the antigen -
> however, FFPE probably will work with human markers. I can also provide
> publications, information about PLP, Becksteads IHC zinc fixative. PLP
> recipes can be found on the internet but I have one that is excellent for
> larger volumes of this fixative. Biocare has information on this fixative
> with their IHC products too.
>
>
>
> Gayle M. Callis
>
> HTL/HT/MT(ASCP)
>
>
>
>
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Sat, 16 Aug 2014 21:05:06 +0800
> From: "E. Wayne Johnson" <ewj <@t> pigsqq.org>
> Subject: Re: [Histonet] RE: Glassware Cleaning again
> To: "Cooper, Brian" <bcooper <@t> chla.usc.edu>, Laurie Colbert
> <lcolbert <@t> pathmdlabs.com>, "Histonet Post
> (histonet <@t> lists.utsouthwestern.edu)"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <53EF5702.1080808 <@t> pigsqq.org>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Bromcresol means Bromocresol.
>
> Faint not, but I certainly can't say you won't dye if you leave out the O.
>
> The "o" is subject to elision due to its difficulty in pronounciation.
>
> Such an occurrence of elision between 2 consonants is called syncope.
>
> In English writing an elided vowel is often replaced with an apostrophe to
> indicate the elision and perhaps demonstrate the dialect of the speaker.
>
> Colloquial dialects in writing are too informal for stuffy scientific
> and medical types, and indeed
> special meaning is liable to be construed to the presence of a spurious
> punctuation in the name of a chemical.
>
> We don't see {brom'cresol} in
> the lab {purple, green} but we can hear of an existence
> where it lives happily under other nomenclature.
>
> So "bromcresol" means "bromocresol".
>
> And as in American politics,
> the removal of an "O" might seem to make a difference visually
> and even might be comfortable to some,
> but actually would amount absolutely nothing in terms of what is
> being represented, or the dying that is going on.
>
>
>
>> On 8/16/2014 12:35 AM, Cooper, Brian wrote:
>> I noticed the discrepancy in spelling too. I looked online for like 30 minutes, and couldn't find anything called "Bromcresol." Found a lot of vendors selling Bromocresol Purple (and Green for that matter). Best I can figure is that this is a typo on CAP's checklist (that's been there for several years now).
>>
>> Thanks,
>>
>> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
>> Department of Pathology and Laboratory Medicine
>> Children's Hospital Los Angeles
>> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
>> bcooper <@t> chla.usc.edu
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
>> Sent: Friday, August 15, 2014 9:13 AM
>> To: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
>> Subject: [Histonet] Glassware Cleaning again
>>
>> Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple.
>>
>> Laurie Colbert, HT (ASCP)
>> Histology Supervisor
>> PATH MD
>> 8158 Beverly Blvd.
>> Los Angeles, CA 90048
>> (323) 648-3214 direct
>> (424) 245-7284 main lab
>>
>> The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message.
>>
>>
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