[Histonet] Tissue falling off

[abtdhu <@t> gmail.com]

Hi Susan,

I was so glad that I saw your post regarding tissue falling off. We have had this problem for a while, especially for human bones tissue. This prevents us to do any heat induced antigen retrieval. If it possible to email me how do you mix soapy water with ammonia? How many ml of water? How much soap and ammonia? I am excited to receive your "recipe" and can not wait to test it.
Thank you very much.

Dorothy Hu
MGH endocrine histocore
abtdhu <@t> gmail.com

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> Tissue that has been properly processed, cut thin and dried well should not be falling off.. We have never needed any additive in our water baths but if we have  tough bones or very bloody tissue we use plus slides. PS: we soak tough or bloody tissue in soapy water with some ammonia as it REALLY makes a difference.
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> Susan Walzer HT(ASCP)
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