[Histonet] Protocol for decalcifying and processing large bone section.

[DiCarlo, Margaret]

I use 10% formic acid for large human bones but I don't think your bone sections are that large.

Peggy DiCarlo
Ortho Bone Lab
BGMC
100 High St. 
Buffalo, NY  14203

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Clough, Bret
Sent: Tuesday, August 05, 2014 11:55
To: Histonet list serv.
Subject: [Histonet] Protocol for decalcifying and processing large bone section.

Hi everyone,
 I was hoping someone on histonet would being willing to help me by either sharing their protocol or advising me on decalcifying and processing large bone sections. The bone sections are from the femural head of sheep measuring roughly 1cm x 1 1/2 cm cube.  Currently I've been decalcifying the samples in 0.5M EDTA which is taking along time to decalcify this being the 19th day.  Any advise would be greatly appreciated!


Sincerely,
   Bret Clough
   Texas A&M Health Science Center
   Temple, TX.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi

CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida HealthÂ’s Technology Assistance Center at (716) 859-7777.