[Histonet] Protocol for decalcifying and processing large bone section.

[Barry Rittman]

I would recommend using Kristensen's "sodium formate/formic acid" mixture.
This is more gentle than formic acid alone and almost as rapid.
Barry


On Tue, Aug 5, 2014 at 12:01 PM, DiCarlo, Margaret <
MDiCarlo <@t> kaleidahealth.org> wrote:

> I use 10% formic acid for large human bones but I don't think your bone
> sections are that large.
>
> Peggy DiCarlo
> Ortho Bone Lab
> BGMC
> 100 High St.
> Buffalo, NY  14203
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Clough, Bret
> Sent: Tuesday, August 05, 2014 11:55
> To: Histonet list serv.
> Subject: [Histonet] Protocol for decalcifying and processing large bone
> section.
>
> Hi everyone,
>  I was hoping someone on histonet would being willing to help me by either
> sharing their protocol or advising me on decalcifying and processing large
> bone sections. The bone sections are from the femural head of sheep
> measuring roughly 1cm x 1 1/2 cm cube.  Currently I've been decalcifying
> the samples in 0.5M EDTA which is taking along time to decalcify this being
> the 19th day.  Any advise would be greatly appreciated!
>
>
> Sincerely,
>    Bret Clough
>    Texas A&M Health Science Center
>    Temple, TX.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The Keeping You Informed section of Kaleida Health`s website features a
> wealth of information, stories and pictures about our valued workforce and
> the tremendous momentum our organization is experiencing. Check us out at:
> www.kaleidahealth.org/kyi
>
> CONFIDENTIALITY NOTICE: This email transmission and any documents, files,
> or previous e-mail messages attached to it are confidential and intended
> solely for the use of the individual or entity to whom they are addressed.
> If you are not the intended recipient, or a person responsible for
> delivering it to the intended recipient, you are hereby notified that any
> further review, disclosure, copying, dissemination, distribution, or use of
> any of the information contained in or attached to this e-mail transmission
> is strictly prohibited. If you have received this message in error, please
> notify the sender immediately by e-mail, discard any paper copies, and
> delete all electronic files of the message. If you are unable to contact
> the sender or you are not sure as to whether you are the intended
> recipient, please call Kaleida HealthÂ’s Technology Assistance Center at (716)
> 859-7777.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>