[Histonet] Protocol for decalcifying and processing large bone section.

[William J. O'Connor III]

Is there a need to decalcify the femoral head intact?  We typically cut large, unfixed veterinary bones with an Exakt band saw into 5 mm slices prior to fixation and decalcification.  This ensures the bones are well fixed prior to decalcification.   We decalcify the selected slices for about 24 hours in hcl or formic acid.
JO'C



-----Original Message-----
From: Clough, Bret <Clough <@t> medicine.tamhsc.edu>
To: Histonet list serv. <histonet <@t> lists.utsouthwestern.edu>
Sent: Tue, Aug 5, 2014 10:56 am
Subject: [Histonet] Protocol for decalcifying and processing large bone section.


Hi everyone,
I was hoping someone on histonet would being willing to help me by either 
haring their protocol or advising me on decalcifying and processing large bone 
ections. The bone sections are from the femural head of sheep measuring roughly 
cm x 1 1/2 cm cube.  Currently I’ve been decalcifying the samples in 0.5M EDTA 
hich is taking along time to decalcify this being the 19th day.  Any advise 
ould be greatly appreciated!

incerely,
  Bret Clough
  Texas A&M Health Science Center
  Temple, TX.
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