[Histonet] Protocol for decalcifying and processing large bone section.

[Rene J Buesa]

As a general rule EDTA is used for the gentle decalcification of small or delicate bones, such as bone marrow samples. For large and dense bones, like the one your are trying to decalcify, you should use a strong acid. In order to prevent having to prepare the decalcifying solution I always used a brand name "RDO".
René J.  


On Tuesday, August 5, 2014 11:55 AM, "Clough, Bret" <Clough <@t> medicine.tamhsc.edu> wrote:
  


Hi everyone,
I was hoping someone on histonet would being willing to help me by either sharing their protocol or advising me on decalcifying and processing large bone sections. The bone sections are from the femural head of sheep measuring roughly 1cm x 1 1/2 cm cube.  Currently I’ve been decalcifying the samples in 0.5M EDTA which is taking along time to decalcify this being the 19th day.  Any advise would be greatly appreciated!


Sincerely,
   Bret Clough
   Texas A&M Health Science Center
   Temple, TX.
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