[Histonet] RE: human cells in rat tissue

[Elizabeth Chlipala]

We have used that too and it will work well in most instances, but there are some issues with that method, detection is entirely dependent upon the number of mitochondria in the cells and some cells contain more than others, as long you understand that you are fine.  We feel we get a better staining reaction with the HLA-1 verses the mitochondria antibody.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-----Original Message-----
From: Mass Histology [mailto:jstaruk <@t> masshistology.com] 
Sent: Thursday, April 17, 2014 11:46 AM
To: Elizabeth Chlipala; 'Marti Gaudes, Merce'; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: human cells in rat tissue

We use an anti-human mitochondria antibody that works well.

Jim

_______________________
James E. Staruk HT(ASCP)
 www.masshistology.com
   www.nehorselabs.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala
Sent: Thursday, April 17, 2014 10:53 AM
To: Marti Gaudes, Merce; Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: human cells in rat tissue

Merce

There is another Human Nuclear antibody from cell signaling we find that it works in FFPE tissues, however in our hands it did not stain all nuclei probably around 90- 95% overall.  We found it to be potentially fixation dependent, we noticed that tissues that had spent longer time in fixative did not stain that well and we also saw some staining in some rat samples, we could not figure out if it was due to our protocol or tissue collection but we decided that there was too many inconsistencies so we did not use it for that particular study.  We might try it again.  We do use another antibody to detect human cells in a rat background and that is HLA-1 the one we use is from abcam.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box
18592
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marti Gaudes, Merce
Sent: Thursday, April 17, 2014 8:41 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] human cells in rat tissue

Hi, good afternoon everyone,

I was wondering if anybody could help me out.  We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue.  I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin.

Do you know any anti human nuclear antibody that works well in paraffin?
Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive...


Thanks a lot!


Mercè Martí Gaudes
Histology Unit

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