[Histonet] RE: human cells in rat tissue

[Mass Histology]

We use an anti-human mitochondria antibody that works well.

Jim

_______________________
James E. Staruk HT(ASCP)
 www.masshistology.com
   www.nehorselabs.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Thursday, April 17, 2014 10:53 AM
To: Marti Gaudes, Merce; Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: human cells in rat tissue

Merce

There is another Human Nuclear antibody from cell signaling we find that it
works in FFPE tissues, however in our hands it did not stain all nuclei
probably around 90- 95% overall.  We found it to be potentially fixation
dependent, we noticed that tissues that had spent longer time in fixative
did not stain that well and we also saw some staining in some rat samples,
we could not figure out if it was due to our protocol or tissue collection
but we decided that there was too many inconsistencies so we did not use it
for that particular study.  We might try it again.  We do use another
antibody to detect human cells in a rat background and that is HLA-1 the one
we use is from abcam.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box
18592
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marti
Gaudes, Merce
Sent: Thursday, April 17, 2014 8:41 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] human cells in rat tissue

Hi, good afternoon everyone,

I was wondering if anybody could help me out.  We would like to detect human
cells engrafted in rat tissue, particularly in formalin-fixed,
paraffin-embedded tissue.  I have so far tried one anti-human nuclear
antigen antibody that works well in criostat...but without good results in
paraffin.

Do you know any anti human nuclear antibody that works well in paraffin?
Or...do you know any other protein that let me differentiate between human
cells and rat tissue? Obviously my cells are not GFP positive...


Thanks a lot!


Mercè Martí Gaudes
Histology Unit

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