[Histonet] RE: human cells in rat tissue

[Elizabeth Chlipala]

Merce

There is another Human Nuclear antibody from cell signaling we find that it works in FFPE tissues, however in our hands it did not stain all nuclei probably around 90- 95% overall.  We found it to be potentially fixation dependent, we noticed that tissues that had spent longer time in fixative did not stain that well and we also saw some staining in some rat samples, we could not figure out if it was due to our protocol or tissue collection but we decided that there was too many inconsistencies so we did not use it for that particular study.  We might try it again.  We do use another antibody to detect human cells in a rat background and that is HLA-1 the one we use is from abcam.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marti Gaudes, Merce
Sent: Thursday, April 17, 2014 8:41 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] human cells in rat tissue

Hi, good afternoon everyone,

I was wondering if anybody could help me out.  We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue.  I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin.

Do you know any anti human nuclear antibody that works well in paraffin? Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive...


Thanks a lot!


Mercè Martí Gaudes
Histology Unit

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