[Histonet] longterm storage of tissue samples in 70% Ethanol

Rene J Buesa rjbuesa <@t> yahoo.com
Wed Sep 18 09:21:25 CDT 2013

Why do you have to store fixed specimens for that long? If you are going to process them anyway, why don't you just process them and store as paraffin blocks? It will be much safer and you can be sure of the antigens preservation.
René J.

From: "Kruttwig, Klaus" <Klaus.Kruttwig <@t> ucsf.edu>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Tuesday, September 17, 2013 6:52 PM
Subject: [Histonet] longterm storage of tissue samples in 70% Ethanol

Dear all,

I have a question about longterm storage of histology samples and was wondering if someone can help me.
I am not sure if my current sample storage system is optimal in terms of antigen- and histology preservation. Do you think it is okay to store 4% paraformaldehyde fixated samples before paraffin embedding for approx 2-3 years in 70% EtOH at 4 degree celsius? Or do I risk a decrease in specimen quality?

Any help will be highly appreciated.


Klaus Kruttwig
Department of Cell and Tissue Biology
University of California - San Francisco
San Franscisco, CA, USA
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