[Histonet] Kisser's Mounting Media (Glycerol jelly)

[Tony Reilly]

Hello

In the dark old days we would seal the edges of the coverslip with nail polish.

Regards
Tony

  

Tony Reilly  B.App.Sc. , M.Sc.

Chief Scientist, Anatomical Pathology

Pathology Queensland-PA Laboratory 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barry Rittman
Sent: Wednesday, 30 October 2013 5:41 AM
To: Tony Reilly
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Kisser's Mounting Media (Glycerol jelly)

You can use thymol or merthiolate to prevent mold.
You can always seal the edges of the coverslip.
Barry



On Tue, Oct 29, 2013 at 1:47 PM, Keyser Gerald T <GKeyser <@t> uwhealth.org>wrote:

> I'm toying with making my own aqueous mounting media. Although I will not
> likely use this in actual cases, it would still be nice. Aqueous mounting
> media is kind of expensive. But, there are some questions that need to be
> answered.
>
> The Slides need to be kept for 10 years.
>
> - Will the adhesive yellow with time?
>
> - Will the adhesive continue to have complete coverage over time. Over
> drying? Cracking?
>
> - Since the mounting media is made from glycerin, gelatin, and water, will
> microbial invasion of the slides be a problem? I can add a anti-microbial
> agent to the recipe.
>
> Anyone have long term experience with this recipe?
>
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