[Histonet] Kisser's Mounting Media (Glycerol jelly)

Barry Rittman barryrittman <@t> gmail.com
Tue Oct 29 14:40:48 CDT 2013


You can use thymol or merthiolate to prevent mold.
You can always seal the edges of the coverslip.
Barry



On Tue, Oct 29, 2013 at 1:47 PM, Keyser Gerald T <GKeyser <@t> uwhealth.org>wrote:

> I'm toying with making my own aqueous mounting media. Although I will not
> likely use this in actual cases, it would still be nice. Aqueous mounting
> media is kind of expensive. But, there are some questions that need to be
> answered.
>
> The Slides need to be kept for 10 years.
>
> - Will the adhesive yellow with time?
>
> - Will the adhesive continue to have complete coverage over time. Over
> drying? Cracking?
>
> - Since the mounting media is made from glycerin, gelatin, and water, will
> microbial invasion of the slides be a problem? I can add a anti-microbial
> agent to the recipe.
>
> Anyone have long term experience with this recipe?
>
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