[Histonet] Kisser's Mounting Media (Glycerol jelly)

[Barry Rittman]

Nail varnish works well
Alternatives include using pink dental wax that melts at relatively low
temperature or a mixture of Vaseline/lanoline/paraffin wax (equal parts
mixed with heat). Both seal well and are impervious to aqueous solutions.
Barry



On Tue, Oct 29, 2013 at 6:01 PM, Tony Reilly
<Tony.Reilly <@t> health.qld.gov.au>wrote:

> Hello
>
> In the dark old days we would seal the edges of the coverslip with nail
> polish.
>
> Regards
> Tony
>
>
>
> Tony Reilly  B.App.Sc. , M.Sc.
>
> Chief Scientist, Anatomical Pathology
>
> Pathology Queensland-PA Laboratory
>
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barry Rittman
> Sent: Wednesday, 30 October 2013 5:41 AM
> To: Tony Reilly
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Kisser's Mounting Media (Glycerol jelly)
>
> You can use thymol or merthiolate to prevent mold.
> You can always seal the edges of the coverslip.
> Barry
>
>
>
> On Tue, Oct 29, 2013 at 1:47 PM, Keyser Gerald T <GKeyser <@t> uwhealth.org
> >wrote:
>
> > I'm toying with making my own aqueous mounting media. Although I will not
> > likely use this in actual cases, it would still be nice. Aqueous mounting
> > media is kind of expensive. But, there are some questions that need to be
> > answered.
> >
> > The Slides need to be kept for 10 years.
> >
> > - Will the adhesive yellow with time?
> >
> > - Will the adhesive continue to have complete coverage over time. Over
> > drying? Cracking?
> >
> > - Since the mounting media is made from glycerin, gelatin, and water,
> will
> > microbial invasion of the slides be a problem? I can add a anti-microbial
> > agent to the recipe.
> >
> > Anyone have long term experience with this recipe?
> >
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