[Histonet] In situ PCR

["E. Wayne Johnson 朱稳森"]

We don't do in-situ PCR, but the principle is that with formalin-fixed 
tissues
your amplified product is trapped in the protein matrix on the slide.




On 3:59, Sarah Dysart wrote:
> Anyone out there do this?  If so, during the PCR step you are amplifying your gene of interest, where does the amplified product go?  Each step of the PCR (from how I am understanding this...I'm new to molecular biology protocols...) separates the double stranded sequence then copies it, and this goes on and on for 30-40 cycles...where does the product go?  Does it just wash off?  If not how is it binding to the tissue?
>
> Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
>
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