[Histonet] In situ PCR

[Sarah Dysart]

Anyone out there do this?  If so, during the PCR step you are amplifying your gene of interest, where does the amplified product go?  Each step of the PCR (from how I am understanding this...I'm new to molecular biology protocols...) separates the double stranded sequence then copies it, and this goes on and on for 30-40 cycles...where does the product go?  Does it just wash off?  If not how is it binding to the tissue?

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912