[Histonet] Masson's Trichrome

[Rene J Buesa]

Just make a quick calculation of how much "diluted" is the cost of the reagent and you will realize that re-using the solutions  is not worth it from the monetary point of view, and much less from the quality aspect. Do not reuse them.
René J.


________________________________
From: "Berger, Rebecca" <BergerR1 <@t> email.chop.edu>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Monday, October 7, 2013 9:53 AM
Subject: [Histonet] Masson's Trichrome


Hi Histonet,
Quick question about Masson's Trichrome. I'm staining several batches of slides by hand, and normally only use the phosphotungstic/phosphomolybdic acid solution, as well as the 1% acetic acid solution, just once and replace it each time I do a stain.  Does anyone out there re-use the acid solution? It seems like a real waste (in many ways) to replace it each time I do the stain but I've yet to experiment with re-using them.
Thanks for your help in advance,
Becky


Rebecca Berger, HT(ASCP)CM
Research Technician
Division of Orthopedic Surgery
The Children's Hospital of Philadelphia Research Institute
3615 Civic Center Boulevard, ARC904 (Lab)
Philadelphia, PA 19104
Phone: 267-425-2076  Email: bergerr1 <@t> email.chop.edu<mailto:bergerr1 <@t> email.chop.edu>
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