Hi all- Has anyone had problems with the salt split method for DIFs not separating? It has been sitting in 1M NaCl for 4 days now and is still not budging. We do not do this method often and any suggestions would be greatly appreciated. Thanks, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo <@t> pathlab.us